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首页> 外文期刊>Journal of Microelectromechanical Systems >A Continuous-Flow Polymerase Chain Reaction Microchip With Regional Velocity Control
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A Continuous-Flow Polymerase Chain Reaction Microchip With Regional Velocity Control

机译：具有区域速度控制的连续流聚合酶链反应芯片

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摘要

This paper presents a continuous-flow polymerase chain reaction (PCR) microchip with a serpentine microchannel of varying width for "regional velocity control." Varying the channel width by incorporating expanding and contracting conduits made it possible to control DNA sample velocities for the optimization of the exposure times of the sample to each temperature phase while minimizing the transitional periods during temperature transitions. A finite element analysis (FEA) and semi-analytical heat transfer model was used to determine the distances between the three heating assemblies that are responsible for creating the denaturation (96℃), hybridization (60℃), and extension (72℃) temperature zones within the microchip. Predictions from the thermal FEA and semi-analytical model were compared with temperature measurements obtained from an infrared (IR) camera. Flow-field FEAs were also performed to predict the velocity distributions in the regions of the expanding and contracting conduits to study the effects of the microchannel geometry on flow recirculation and bubble nucleation. The flow fields were empirically studied using micro particle image velocimetry (μ-PIV) to validate the flow-field FEA's and to determine experimental velocities in each of the regions of different width. Successful amplification of a 90 base pair (bp) bacillus anthracis DNA fragment was achieved.
机译：本文提出了一种具有可变宽度的蛇形微通道的连续流动聚合酶链反应（PCR）微芯片，用于“区域速度控制”。通过结合膨胀和收缩导管来改变通道宽度，可以控制DNA样品的速度，以优化样品在每个温度阶段的暴露时间，同时最小化温度转换过程中的转换时间。使用有限元分析（FEA）和半解析热传递模型来确定负责引起变性（96℃），杂交（60℃）和延伸（72℃）温度的三个加热组件之间的距离。微芯片内的区域。将热FEA和半分析模型的预测结果与从红外（IR）摄像机获得的温度测量结果进行了比较。还进行了流场有限元分析以预测膨胀和收缩管道区域中的速度分布，以研究微通道几何形状对流动再循环和气泡成核的影响。使用微粒图像测速仪（μ-PIV）对流场进行了经验研究，以验证流场FEA并确定不同宽度的每个区域中的实验速度。成功扩增了一个90个碱基对（bp）的炭疽杆菌DNA片段。

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