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Microvalves for integrated selective droplet generation, splitting and merging on a chip

机译:用于集成的选择性液滴生成,分裂和合并芯片的微型阀

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In this paper, we demonstrate a selective multisite splitting and merging system to perform the type of permutations of reactions required for high-throughput screening. Our approach uses multiple single-layer microfluidic valves as they offer simplicity, biocompatibility, and high-speed operation. These valves are used both to control the generation of droplets and the location of splitting. In the former, the valves are used to completely block some microchannels; in the latter, they create a more modest local geometry variation. The generated droplets act as a vessel that transports different samples around the chip; these vessels can then be subdivided at splitting locations, just as a pipette can be used to dispense a fraction of its total volume. The split droplets enter a merging channel, in which daughter droplets of different types can be merged. We demonstrate the merging of different droplet types in different concentrations at four merging locations.
机译:在本文中,我们展示了一种选择性多路分裂和合并系统,以执行高通量筛选所需的反应所需的类型。 我们的方法使用多层微流体阀,因为它们提供了简单,生物相容性和高速操作。 这些阀门用于控制液滴的产生和分裂的位置。 在前者中,阀门用于完全阻挡一些微通道; 在后者中,它们创建了更适度的本地几何变化。 所产生的液滴充当芯片围绕芯片的不同样品的血管; 然后可以将这些血管细分在分裂位置,就像吸移管一样可用于分配其总体积的一小部分。 分割液滴进入合并通道,可以合并不同类型的子液滴。 我们在四个合并位置展示了不同浓度的不同液滴类型的合并。

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