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首页> 外文期刊>Molecular Biology Reports >Characterization and expression analysis of the myeloid differentiation factor 88 (MyD88) in rock bream Oplegnathus fasciatus
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Characterization and expression analysis of the myeloid differentiation factor 88 (MyD88) in rock bream Oplegnathus fasciatus

机译：岩鲷Oplegnathus fasciatus髓系分化因子88（MyD88）的表征和表达分析

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摘要

Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein able to activate nuclear factor-kappa B (NF-κB) through interactions with interleukin-1 receptor (IL-1R) and the Toll-like receptors (TLRs), with the exception of TLR3. Here, we describe the identification of MyD88 from the rock bream fish Oplegnathus fasciatus and its characterization based on GS-FLX™ sequencing. The cDNA of rock bream MyD88 was found to be composed of 1626 bp, with an 867 bp open reading frame that encodes 288 amino acids. The deduced amino acid sequence of MyD88 possessed both a conserved death domain at the amino terminus and a typical Toll-IL-1 receptor (TIR) domain at the carboxyl terminus, similar to that found in other fishes, amphibians, avians, mammals and invertebrates. The mRNA expression pattern of MyD88 in healthy and bacterially challenged rock bream were examined using quantitative real-time polymerase chain reaction (qRT-PCR). MyD88 transcripts were found to be strongly expressed in blood, gill, liver, spleen, head kidney and kidney, moderately expressed in skin, brain and intestine, and weakly expressed in muscle. Expression levels of MyD88 in blood, spleen and head kidney were dramatically up-regulated upon exposure to LPS and the Gram-negative bacteria Edwardsiella tarda, suggesting that MyD88 plays an important role in rock bream defenses against bacterial infection.
机译：髓样分化因子88（MyD88）是一种通用衔接蛋白，能够通过与白介素1受体（IL-1R）和Toll样受体（TLR）相互作用来激活核因子-κB（NF-κB）。 TLR3。在这里，我们描述了从鱼Oplegnathus fasciatus鉴定MyD88及其基于GS-FLX™测序的表征。发现rock鲷MyD88的cDNA由1626 bp组成，具有867 bp的开放阅读框，编码288个氨基酸。推导的MyD88氨基酸序列在氨基末端具有一个保守的死亡结构域，在羧基末端具有一个典型的Toll-IL-1受体（TIR）结构域，与在其他鱼类，两栖动物，禽类，哺乳动物和无脊椎动物中发现的相似。使用定量实时聚合酶链反应（qRT-PCR）检测了健康和细菌攻击的鲷鱼中MyD88的mRNA表达模式。发现MyD88转录本在血液，腮，肝，脾，头肾和肾脏中强烈表达，在皮肤，脑和肠中中等表达，而在肌肉中弱表达。暴露于LPS和革兰氏阴性细菌爱德华氏菌后，血液，脾脏和头部肾脏中MyD88的表达水平显着上调，这表明MyD88在岩鲷防御细菌感染中起着重要作用。

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