...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Biology Reports >Cloning and expression study of a putative carotene biosynthesis related (cbr) gene from the halotolerant green alga Dunaliella salina
【24h】

Cloning and expression study of a putative carotene biosynthesis related (cbr) gene from the halotolerant green alga Dunaliella salina

机译：耐盐绿色藻杜氏盐藻中假定的胡萝卜素生物合成相关（cbr）基因的克隆和表达研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dunaliella salina, a unicelluar green alga that can tolerate an extreme variation of salt concentration is being studied as a model system to analyze the tolerance of abiotic stresses at the molecular level. Upon abnormal NaCl levels, new transcripts were abundantly expressed in cells of the alga. EST gene discovery efforts utilizing salt-shock cells had identified one cDNA designated Dscbr (GenBank accession no. DQ867041) with significant similarity to a carotene biosynthesis related gene (cbr) from Dunaliella bardawil and to early light inducible genes (elip) of higher plants. Dscbr was 976 bp in length, encoding a 190 amino acid deduced polypeptide (DsCBR) with a predicted molecular mass of 19.9 kDa and pI of 9.0. The three dimensional structure of DsCBR modeled by computer homology modeling techniques showed that the protein possessed three predicted transmembrane helices and six conserved pigment-binding residues. Real-Time Quantitative PCR clearly demonstrated that Dscbr mRNA can be rapidly induced by high light intensity and salt shocks. The results presented in this work are consistent with the earlier proposal (Jin et al. 2001 Biochim Biophys Acta 1506:244–259, 2003 Plant Physiol 132:352–364) that the DsCBR protein is an adaptive response to stress-induced photodamage within the alga chloroplast, and plays a key role in the protection and/or repair of the photosynthetic apparatus.
机译：作为能在分子水平上分析非生物胁迫耐受性的模型系统，正在研究杜氏盐藻（Dunaliella salina）（一种能容忍盐浓度极端变化的单细胞绿藻）。当NaCl水平异常时，新的转录本在藻类细胞中大量表达。利用盐冲击细胞进行的EST基因发现工作已经鉴定出一种名为Dscbr的cDNA（GenBank登录号DQ867041），该cDNA与来自杜氏杜氏藻的胡萝卜素生物合成相关基因（cbr）和高等植物的早期光诱导基因（elip）极为相似。 Dscbr的长度为976 bp，编码190个氨基酸推导的多肽（DsCBR），预测的分子量为19.9 kDa，pI为9.0。通过计算机同源建模技术建模的DsCBR的三维结构表明，该蛋白具有三个预测的跨膜螺旋和六个保守的色素结合残基。实时定量PCR清楚地表明，高光强度和盐冲击可快速诱导Dscbr mRNA。这项工作中提出的结果与先前的建议（Jin等人，2001 Biochim Biophys Acta 1506：244-259，2003 Plant Physiol 132：352-364）一致，DsCBR蛋白是对内源性胁迫诱导的光损伤的适应性反应。藻类叶绿体，并在保护和/或修复光合装置中起关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号