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首页> 外文期刊>Molecular BioSystems >Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae
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Phenomic and transcriptomic analyses reveal that autophagy plays a major role in desiccation tolerance in Saccharomyces cerevisiae

机译：形态学和转录组学分析表明自噬在酿酒酵母的耐干燥性中起主要作用

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摘要

Saccharomyces cerevisiae can survive extreme desiccation, but the molecular mechanisms are poorly understood. To define genes involved in desiccation tolerance, two complementary genome-wide approaches, phenomics and transcriptomics, have been used, together with a targeted analysis of gene deletion mutants tested individually for their ability to survive drying. Genome-wide phenotypic analyses carried out on a pooled library of single-gene deletion mutants subjected to three cycles of desiccation and re-growth to post-diauxic phase identified about 650 genes that contributed to strain survival in the drying process. Air-drying desiccation-tolerant post-diauxic phase cells significantly altered transcription in 12% of the yeast genome, activating expression of over 450 genes and down-regulating 330. Autophagy processes were significantly over-represented in both the phenomics study and the genes up-regulated on drying, indicating the importance of the clearance of protein aggregates/damaged organelles and the recycling of nutrients for the survival of desiccation in yeast. Functional carbon source sensing networks governed by the PKA, Tor and Snfl protein kinase complexes were important for the survival of desiccation, as indicated by phenomics, transcriptomics, and individual analyses of mutant strains. Changes in nitrogen metabolism were evident during the drying process and parts of the environmental stress response were activated, repressing ribosome production and inducing genes for coping with oxidative and osmotic stress.
机译：酿酒酵母可以在极端干燥中幸存下来，但是其分子机制尚不清楚。为了定义参与干燥耐性的基因，已经使用了两种互补的全基因组方法，即表观学和转录组学，以及针对基因缺失突变体的针对其存活干燥能力的针对性分析。对单基因缺失突变体的库进行基因组范围的表型分析，该突变体经历了三个循环的干燥和重新生长至双生后阶段，确定了约650个基因在干燥过程中有助于菌株存活。风干的耐干燥后双相期细胞显着改变了酵母基因组中12％的转录，激活了450多个基因的表达并下调了330个基因。自噬过程在基因组学研究和基因上显着-调节干燥，表明清除蛋白质聚集体/受损细胞器以及循环利用营养物质对于酵母中干燥的存活至关重要。由表型，转录组学和突变株的个体分析表明，由PKA，Tor和Snf1蛋白激酶复合物控制的功能性碳源传感网络对于干燥的存活很重要。在干燥过程中氮代谢的变化很明显，部分环境胁迫反应被激活，抑制了核糖体的产生并诱导了应对氧化和渗透胁迫的基因。

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  • 来源
    《Molecular BioSystems》 |2011年第1期|p.139-149|共11页
  • 作者

    Sooraj Ratnakumar; Andy Hesketh; Konstantinos Gkargkas; Michael Wilson; Bharat M. Rash; Andrew Hayes; Alan Tunnacliffe; Stephen G. Oliver;

  • 作者单位

    Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT.UK;

    Cambridge Systems Biology Centre & Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK;

    Cambridge Systems Biology Centre & Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK;

    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK,The Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK;

    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK;

    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK;

    Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT.UK;

    Cambridge Systems Biology Centre & Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK,Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK;

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