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首页> 外文期刊>Molecular Biotechnology >cDNA-AFLP Analysis Reveals Differential Gene Expression in Response to Salt Stress in Foxtail Millet (Setaria italica L.)
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cDNA-AFLP Analysis Reveals Differential Gene Expression in Response to Salt Stress in Foxtail Millet (Setaria italica L.)

机译：cDNA-AFLP分析揭示了谷子（Setaria italica L.）对盐胁迫的响应差异基因表达。

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Plant growth and productivity are affected by various abiotic stresses such as heat, drought, cold, salinity, etc. The mechanism of salt tolerance is one of the most important subjects in plant science as salt stress decreases worldwide agricultural production. In our present study we used cDNA-AFLP technique to compare gene expression profiles of a salt tolerant and a salt-sensitive cultivar of foxtail millet (Seteria italica) in response to salt stress to identify early responsive differentially expressed transcripts accumulated upon salt stress and validate the obtained result through quantitative real-time PCR (qRT-PCR). The expression profile was compared between a salt tolerant (Prasad) and susceptible variety (Lepakshi) of foxtail millet in both control condition (L0 and P0) and after 1 h (L1 and P1) of salt stress. We identified 90 transcript-derived fragments (TDFs) that are differentially expressed, out of which 86 TDFs were classified on the basis of their either complete presence or absence (qualitative variants) and 4 on differential expression pattern levels (quantitative variants) in the two varieties. Finally, we identified 27 non-redundant differentially expressed cDNAs that are unique to salt tolerant variety which represent different groups of genes involved in metabolism, cellular transport, cell signaling, transcriptional regulation, mRNA splicing, seed development and storage, etc. The expression patterns of seven out of nine such genes showed a significant increase of differential expression in tolerant variety after 1 h of salt stress in comparison to salt-sensitive variety as analyzed by qRT-PCR. The direct and indirect relationship of identified TDFs with salinity tolerance mechanism is discussed.
机译：植物的生长和生产力受各种非生物胁迫的影响，例如高温，干旱，寒冷，盐度等。耐盐性机制是植物科学中最重要的课题之一，因为盐胁迫降低了全球农业产量。在我们目前的研究中，我们使用cDNA-AFLP技术比较了盐胁迫下狐尾粟（Seteria italica）的耐盐和耐盐品种的基因表达谱，以鉴定在盐胁迫下积累的早期响应差异表达转录本并进行验证通过定量实时PCR（qRT-PCR）获得的结果。比较了在对照条件（L0 和P0 ）和1 h（L1 和P1）后谷子的耐盐性（Prasad）和敏感品种（Lepakshi）的表达谱）。我们鉴定了90个差异表达的转录本片段（TDF），其中86个TDF根据其完全存在或不存在（定性变体）和4个差异表达模式水平（定量变体）进行了分类品种。最后，我们鉴定了27种非冗余的差异表达cDNA，它们是耐盐品种所特有的，它们代表参与代谢，细胞转运，细胞信号转导，转录调控，mRNA剪接，种子发育和贮藏等的不同基因组。表达模式通过qRT-PCR分析，与盐敏感品种相比，九个此类基因中的七个基因在盐胁迫1 h后的耐性品种中差异表达显着增加。讨论了鉴定出的TDF与盐分耐受机制的直接和间接关系。

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