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首页> 外文期刊>Biochemical and Biophysical Research Communications >Comparative transcriptome analysis of differentially expressed genes in foxtail millet (Setaria italica L.) during dehydration stress.
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Comparative transcriptome analysis of differentially expressed genes in foxtail millet (Setaria italica L.) during dehydration stress.

机译：脱水胁迫下谷子（Setaria italica L.）差异表达基因的比较转录组分析。

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摘要

Dehydration stress is one of the most important abiotic stresses that adversely influence crop growth and productivity. With the aim to understand the molecular mechanisms underlying dehydration stress tolerance in foxtail millet (Setaria italica L.), a drought tolerant crop, we examined its transcriptome changes at two time points (early and late) of dehydration stress. Two suppression subtractive hybridization (SSH) forward libraries were constructed from 21-day old seedlings of tolerant cv. Prasad at 0.5 and 6h PEG-induced dehydration stress. A total of 327 unique ESTs were identified from both libraries and were classified into 11 different categories according to their putative functions. The plant response against dehydration stress was complex, representing major transcripts involved in metabolism, stress, signaling, transcription regulation, translation and proteolysis. By Reverse Northern (RN) technique we identified the differential expression pattern of 327 transcripts, 86 (about 26%) of which showed > or = 1.7-fold induction. Further the obtained results were validated by quantitative real-time PCR (qRT-PCR) to have a comparative expression profiling of randomly chosen 9 up-regulated transcripts (> or =2.5 fold induction) between cv. Prasad (tolerant) and cv. Lepakshi (sensitive) upon dehydration stress. These transcripts showed a differential expression pattern in both cultivars at different time points of stress treatment as analyzed by qRT-PCR. The possible relationship of the identified transcripts with dehydration tolerance mechanism is discussed.
机译：脱水胁迫是最重要的非生物胁迫之一，会对作物生长和生产力产生不利影响。为了了解耐旱作物谷子（Setaria italica L.）的脱水胁迫耐受性的分子机制，我们在脱水胁迫的两个时间点（早期和晚期）检查了其转录组变化。从耐受性cv的21日龄幼苗中构建了两个抑制性消减杂交（SSH）正向文库。 Prasad在0.5和6h PEG诱导的脱水压力下。从这两个库中总共鉴定出327个独特的EST，并根据其假定功能将其分为11个不同的类别。植物对脱水胁迫的反应是复杂的，代表涉及代谢，胁迫，信号传导，转录调节，翻译和蛋白水解的主要转录本。通过反向Northern（RN）技术，我们鉴定了327个转录本的差异表达模式，其中86个（约26％）显示出>或= 1.7倍的诱导率。此外，通过定量实时PCR（qRT-PCR）验证了获得的结果，以在cv之间具有随机选择的9种上调转录本（>或= 2.5倍诱导）的比较表达谱。 Prasad（宽容）和简历。 Lepakshi（敏感）在脱水压力下。如通过qRT-PCR分析的，这些转录物在两个品种的胁迫处理的不同时间点显示差异表达模式。讨论了鉴定出的转录本与脱水耐受机制的可能关系。

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