...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Breeding >Development of single nucleotide polymorphism (SNP) markers for use in commercial maize (Zea mays L.) germplasm
【24h】

Development of single nucleotide polymorphism (SNP) markers for use in commercial maize (Zea mays L.) germplasm

机译：开发用于商业玉米（Zea mays L.）种质的单核苷酸多态性（SNP）标记

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The development of single nucleotide polymorphism (SNP) markers in maize offers the opportunity to utilize DNA markers in many new areas of population genetics, gene discovery, plant breeding and germplasm identification. However, the steps from sequencing and SNP discovery to SNP marker design and validation are lengthy and expensive. Access to a set of validated SNP markers is a significant advantage to maize researchers who wish to apply SNPs in scientific inquiry. We mined 1,088 loci sequenced across 60 public inbreds that have been used in maize breeding in North America and Europe. We then selected 640 SNPs using generalized marker design criteria that enable utilization with several SNP chemistries. While SNPs were found on average every 43 bases in 1,088 maize gene sequences, SNPs that were amenable to marker design were found on average every 623 bases; representing only 7% of the total SNPs discovered. We also describe the development of a 768 marker multiplex assay for use on the Illumina? BeadArray? platform. SNP markers were mapped on the IBM2 intermated B73 × Mo17 high resolution genetic map using either the IBM2 segregating population, or segregation in multiple parent-progeny triplets. A high degree of colinearity was found with the genetic nested association map. For each SNP presented we give information on map location, polymorphism rates in different heterotic groups and performance on the Illumina? platform.
机译：玉米中单核苷酸多态性（SNP）标记的发展提供了在群体遗传学，基因发现，植物育种和种质鉴定等许多新领域中利用DNA标记的机会。但是，从测序和SNP发现到SNP标记设计和验证的步骤既漫长又昂贵。对于希望在科学探究中应用SNP的玉米研究人员而言，获得一组经过验证的SNP标记是一项重大优势。我们从60个公共近交系中提取了1,088个基因座，这些近交系已用于北美和欧洲的玉米育种。然后，我们使用通用标记设计标准选择了640个SNP，这些标准可用于多种SNP化学。在1088个玉米基因序列中，平均每43个碱基就有一个SNP，而平均每623个碱基中就有一个适合标记设计的SNP。仅占发现的SNP总数的7％。我们还描述了在Illumina？ BeadArray？上使用的768标记多重分析的开发。平台。使用IBM2隔离种群或在多个亲子后代三胞胎中进行隔离，将SNP标记定位在IBM2所确定的B73×Mo17高分辨率遗传图谱上。遗传嵌套关联图发现高度共线性。对于给出的每个SNP，我们都会提供有关地图位置，不同杂合基团中的多态性率以及Illumina？平台上的性能的信息。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号