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首页> 外文期刊>Molecular Breeding >A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers
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A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

机译：基于单剂量分子标记的四倍体Paspalum notatumFlügge（bahiagrass）遗传图谱

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摘要

Paspalum notatum Flügge is a warm-season forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representatives. Diploid races reproduce sexually and require crosspollination due to a self-incompatible mating system, while autotetraploids reproduce by aposporous apomixis. The objectives of this work were to develop a genetic linkage map of Paspalum notatum Flügge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossing a synthetic completely sexual tetraploid plant (Q4188) as female parent with a natural aposporous individual (Q4117) as pollen donor. Map construction was based on single-dose markers (SDAFs) segregating from both parents. Two linkage maps (female and male) were constructed. Within each map, homologous groups were assembled by detecting repulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose markers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome coverage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps, respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombination and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory.
机译：Paspalum notatumFlügge是一种暖季型牧草，主要代表二倍体（2n = 20）和同四倍体（2n = 40）。由于自交不亲和的交配系统，二倍体种族有性繁殖并需要异花授粉，而同四倍体则通过无孔无融合生殖繁殖。这项工作的目的是在四倍体水平上绘制Paspalum notatumFlügge的遗传连锁图谱，鉴定与无融合生殖相关的连锁群，并对其遗传模式进行一般性表征。通过将作为雌性亲本的合成的完全有性四倍体植物（Q4188）与作为花粉供体的天然无孔个体（Q4117）杂交，获得了一个以繁殖方式分离的113个个体的假试验杂交F1 家族。地图的构建基于与父母双方分开的单剂量标记（SDAF）。绘制了两个链接图（女性和男性）。在每个图谱中，通过检测排斥相链接的SDAF组装同源基团。推定的Q4188和Q4117同源物组通过绘制共享单剂量标记（BSDF）进行鉴定。 Q4188图谱包含263个标记，分布在26个共同分离的群体上，总遗传距离为1.590.6 cM，而Q4117图谱包含216个基因座，分布在39个共同分离的群体上，沿2.265.7 cM分布，从而提供了估计的基因组覆盖范围分别为88％和83％。在Q4188和Q4117图谱中分别检测到7条和12条推定的同源染色体。之后，通过定位BSDFs鉴定出十个雌性和雄性同源染色体。在Q4117图中，单个连接基团与子孢相关。它的特点是重组受限和优先染色体配对。该组中的BPSD标记图谱可检测出雌性同系物和推定的带有单孔的假定的四个雄性组。

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