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首页> 外文期刊>Molecular Human Reproduction >Increased expression of latent TGF-? binding protein-1 and fibrillin-1 in human uterine leiomyomata
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Increased expression of latent TGF-? binding protein-1 and fibrillin-1 in human uterine leiomyomata

机译：潜在TGF-β的表达增加子宫平滑肌瘤中的结合蛋白-1和原纤维蛋白-1

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We compared latent TGF-? binding protein-1 (LTBP-1) and fibrillin-1 (FBN-1) expression in leiomyomata and myometrium, correlated with leiomyomata size. We studied in vivo and in vitro effects of ovarian steroids using matched leiomyomata and myometrium samples from both phases of the menstrual cycle. Leiomyomata were divided into small (≤2 cm), medium (3–5 cm) and large (≥6 cm) groups. We validated LTBP-1 and FBN-1 expression using QPCR, western blot and immunohistochemistry. LTBP-1 and FBN-1 mRNA and protein expressions were higher in the medium-sized group compared with myometrium in the proliferative phase (P = 0.01; P = 0.01). FBN-1 mRNA expression was higher in the secretory phase (P = 0.01). LTBP-1 mRNA and protein expression was higher in the medium group compared with the small and large groups in the proliferative phase (P = 0.04; P = 0.04). No differences between groups were seen in FBN-1 expression in either phase. 17β-eestradiol (E2) increased mRNA and protein expression of LTBP-1 and FBN-1 in cultured leiomyoma smooth muscle cells (LSMC) (P < 0.05). No change in FBN-1 and LTBP-1 expression was observed when cells were treated with E2 plus progesterone. Estrogen may be involved in LTBP-1 and FBN-1 expression in leiomyomata. Extracellular matrix metabolism may be different in medium-sized leiomyoma.
机译：我们比较了潜在的TGF-？平滑肌瘤和子宫肌层中结合蛋白-1（LTBP-1）和原纤维蛋白-1（FBN-1）的表达与平滑肌瘤的大小有关。我们使用月经周期两个阶段的匹配的平滑肌瘤和子宫肌层样本研究了卵巢类固醇的体内和体外作用。平滑肌瘤分为小（≤2cm），中（3–5 cm）和大（≥6cm）组。我们使用QPCR，蛋白质印迹和免疫组化方法验证了LTBP-1和FBN-1的表达。在增生期，中型组的LTBP-1和FBN-1的mRNA和蛋白表达高于子宫内膜（P = 0.01； P = 0.01）。在分泌期，FBN-1 mRNA表达较高（P = 0.01）。在增殖期，中型组的LTBP-1 mRNA和蛋白表达高于小组和大组（P = 0.04； P = 0.04）。在两个阶段中，FBN-1表达均未见组间差异。 17β-雌二醇（E 2 ）在培养的平滑肌瘤平滑肌细胞（LSMC）中提高了LTBP-1和FBN-1的mRNA和蛋白表达（P <0.05）。用E 2 加孕酮处理细胞后，未观察到FBN-1和LTBP-1表达的变化。雌激素可能参与平滑肌瘤中LTBP-1和FBN-1的表达。中型平滑肌瘤的细胞外基质代谢可能不同。

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