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首页> 外文期刊>Mycotoxin Research >Alternaria toxins alternariol and alternariol monomethyl ether in grain foods in Canada
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Alternaria toxins alternariol and alternariol monomethyl ether in grain foods in Canada

机译：加拿大谷物食品中的链霉菌毒素链霉菌毒素和链烷醇单甲醚

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Alternaria alternata has been reported to be the most common fungus on Canadian Western wheat. The Alternaria toxins alternariol (AOH) and alternariol monomethyl ether (AME) are mutagenic in vitro and there is also limited evidence for carcinogenic properties. They have been found in wheat from Europe, Argentina, China and Australia, but they have not been looked for in Canadian grains or grain foods. In the present study, 83 samples of grain-based food sold in Canada, including flour, bran, breakfast cereals, infant cereals and bread, were analysed for AOH and AME using extraction with methanol, clean-up on combined aminopropyl/C18 solid phase extraction (SPE) columns, and liquid chromatography (LC) with tandem mass spectrometric (MS/MS) determination. The overall average recoveries of AOH and AME from a variety of spiked cereal foods (n = 13) were 45 ± 9 % and 53 ± 9 %, which could be attributed mainly to MS matrix effects The instrumental limits of detection (LOD) were 0.34 ng/g and 0.13 ng/g for AOH and AME, respectively, and the instrumental limits of quantitation (LOQ) were 1.1 and 0.43 ng/g. Of 83 samples analysed, 70 were positive for AOH (up to 63 ng/g, in a soft wheat bran) and 64 contained AME (up to 12 ng/g in a bran-based breakfast cereal). Of particular interest was the presence of AOH and/or AME in 27 out of 30 infant foods (up to 4.4 ng/g and 9.0 ng/g, respectively, in a sample of multigrain cereal).
机译：据报道，链格孢菌是加拿大西部小麦中最常见的真菌。链格孢菌毒素链格孢菌（AOH）和链格孢菌单甲醚（AME）在体外具有致突变性，并且关于致癌性的证据也很少。在欧洲，阿根廷，中国和澳大利亚的小麦中都发现了它们，但加拿大谷物或谷类食品中却没有发现它们。在本研究中，使用甲醇萃取并结合氨基丙基/ C18固相进行净化，分析了加拿大销售的83种谷物食品样品，包括面粉，麸皮，早餐谷物，婴儿谷物和面包，以分析AOH和AME。萃取（SPE）柱，以及液相色谱（LC）和串联质谱（MS / MS）测定。从各种加标谷物食品（n = 13）中，AOH和AME的总体平均回收率分别为45±9％和53±9％，这主要归因于MS基质效应。检测限（LOD）为0.34 AOH和AME分别为ng / g和0.13 ng / g，仪器的定量限（LOQ）为1.1和0.43 ng / g。在分析的83个样本中，有70个的AOH呈阳性（在软麦麸中高达63 ng / g）和64个包含AME（在以麸皮为基础的早餐谷物中高达12 ng / g）。特别令人感兴趣的是30种婴儿食品中有27种存在AOH和/或AME（杂粮谷物样品中分别高达4.4 ng / g和9.0 ng / g）。

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