...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mycotoxin Research >Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry
【24h】

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

机译：亲水相互作用色谱-串联质谱法测定谷物中的脱氧雪腐酚磺酸盐（DONS）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Deoxynivalenol (DON) is one of most widespread mycotoxins in cereal commodities, and animal feed is prevalently contaminated at high concentrations. This poses a problem in animal nutrition as especially pigs are very sensitive to DON. An effective process for the reduction of the DON concentration is the treatment of contaminated feed with sodium bisulfite (SBS) whereby DON is transformed into DON-sulfonate (DONS). Although the success of this treatment has been confirmed in several feeding studies, it is unexplained if the decrease of DON is accompanied with a coincident increase of DONS. For this reason, we developed a method for the analysis of DONS using hydrophilic interaction chromatography coupled to tandem mass spectrometry. In order to investigate the correlation between DON and DONS concentrations during SBS-treatment, DON-contaminated wheat was treated with SBS and stored for up to 36 days. At defined timepoints of this treatment, samples were analyzed for DON and DONS using stable isotope labeled standards. The preparation, purification, and structure elucidation of DONS, and the HILIC-HPLC-MS/MS method for the analysis of DONS as well as the results of two storage experiments are presented in this paper.
机译：脱氧雪腐酚（DON）是谷物商品中最广泛的霉菌毒素之一，动物饲料普遍被高浓度污染。这给动物营养带来了问题，因为特别是猪对DON非常敏感。降低DON浓度的有效方法是用亚硫酸氢钠（SBS）处理受污染的饲料，从而将DON转化为DON-磺酸盐（DONS）。尽管在一些喂养研究中已经证实了这种治疗方法的成功，但尚不能解释DON的减少是否伴随DONS的同时增加。因此，我们开发了一种使用亲水相互作用色谱和串联质谱分析DONS的方法。为了调查SBS处理期间DON和DONS浓度之间的相关性，用SBS处理受DON污染的小麦，并保存长达36天。在此处理的指定时间点，使用稳定的同位素标记标准物分析样品中的DON和DONS。本文介绍了DONS的制备，纯化和结构解析，以及用于DONS分析的HILIC-HPLC-MS / MS方法以及两次储存实验的结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号