• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>NanoBioscience, IEEE Transactions on >Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5- Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line
【24h】

Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5- Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line

机译：评估PLGA包覆的氧化铁纳米粒子作为5-氟尿嘧啶载体和兆电压X射线辐射对DU145前列腺癌细胞系的细胞毒作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study was to investigate the uptake and cytotoxic effects of magnetic poly lactic-co-glycolic acid (PLGA)-coated iron oxide nanoparticles as a carrier of 5-fluorouracil (5-FU) and X-ray on the level of proliferation capacity of DU145 prostate carcinoma cell line in monolayer culture. Following monolayer culture, DU 145 cells were treated with different concentrations of 5-FU or 5-FU loaded nanoparticles for 24 h and 2Gy X-ray (6 Mega-voltage (MV)). The rate of nanoparticles penetration was then measured using atomic adsorption spectroscopy (AAS). The cytotoxicity effect of these nanoparticles with/ without X-ray radiation was evaluated using colony formation assay. Spectroscopy results showed that iron content and therefore the cellular uptake of 5-FU loaded nanoparticles increased with increasing nanoparticle concentrations. Further, the proliferation capacity of the cells decreased with the increase of 5-FU and 5- FU loaded nanoparticle concentrations in combination with X-ray radiation. However the extent of reduction in colony number following treatment with 5-FU-loaded nanoparticles in combination with 2Gy of megavoltage X-ray radiation was significantly more than for free 5-FU. Thus, drug-loaded nanoparticles could deliver 5-FU more efficiently into the cells. PLGA coated iron oxide nanoparticles are therefore effective drug delivery vehicles for 5-FU. PLGA coated iron oxide nanoparticles are biocompatible and this coating is an appropriate surface that can penetrate into the cells.
机译：这项研究的目的是研究磁性聚乳酸-乙醇酸共聚物（PLGA）包覆的氧化铁纳米颗粒作为5-氟尿嘧啶（5-FU）和X射线载体的吸收和细胞毒性作用，并研究单层培养中DU145前列腺癌细胞系的增殖能力单层培养后，用不同浓度的5-FU或5-FU负载的纳米颗粒处理DU 145细胞24小时和2Gy X射线（6兆伏（MV））。然后使用原子吸收光谱法（AAS）测量纳米颗粒的渗透速率。使用集落形成测定法评估了具有/不具有X射线辐射的这些纳米颗粒的细胞毒性作用。光谱结果表明，铁含量，因此5-FU负载的纳米颗粒的细胞吸收随着纳米颗粒浓度的增加而增加。此外，细胞的增殖能力随着结合X射线辐射的5-FU和5-FU负载的纳米颗粒浓度的增加而降低。但是，用载有5-FU的纳米颗粒与2Gy的兆伏X射线辐射联合处理后，菌落数量减少的程度明显大于游离5-FU。因此，载有药物的纳米颗粒可以更有效地将5-FU递送到细胞中。因此，PLGA涂层的氧化铁纳米颗粒是用于5-FU的有效药物递送载体。 PLGA涂层的氧化铁纳米颗粒具有生物相容性，该涂层是可以渗入细胞的合适表面。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号