• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Characterization and evaluation of surface modified superparamagnetic iron oxide nanoparticles for uptake into human prostate carcinoma cells.
【24h】

Characterization and evaluation of surface modified superparamagnetic iron oxide nanoparticles for uptake into human prostate carcinoma cells.

机译：表面修饰的超顺磁性氧化铁纳米粒子对人前列腺癌细胞摄取的表征和评估。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Magnetic nanoparticles (MNPs) were synthesized from Fe2+ and Fe3+ by the co-precipitation method. Two nanoparticle surface coatings (gum arabic and sodium citrate) provided additional functionalization and cell selectivity. The incorporation of surface modifiers to the synthesized nanoparticles generated three nanoparticle systems from which the research was based. The physical and chemical properties were determined with a variety of standard characterization techniques. Upon characterization of the nanoparticles, in vitro cell culture experiments were conducted. Nanoparticles were allowed to co-exist with prostate cells for 48 hours under sterile conditions. A range of iron oxide nanoparticle concentrations (0.5 x 1016 -- 50 x 1016 particles/mL) were studied and compared for their effects on cell viability, intercellular uptake and quantitative analysis of prostate cell selectivity.;Nanoparticles have a natural tendency to agglomerate; therefore, the addition of gum arabic as a stabilizing agent provided increased electrostatic repulsion which freely dispersed the particles and greatly contributed to more stabilized particles in both water and cell nutrient media. Sodium citrate was an excellent capping agent for nanoparticles in water but not in serum rich nutrient media. The contents of the media contributed to the destabilization of the nanoparticle solution; hence, particle size measurements decreased over time for sodium citrate coated particles in media as well as non-coated nanoparticles in media, which formed larger particles (350 -- 375 nm), then gradually fell out of solution. Initial measurements for sodium citrate MNPs were more than four times greater in media as compared to stabilized sodium citrate particles in water (160 nm). Non-coated nanoparticles demonstrated agglomeration in water and size measurements increased to 700nm over the 48 hour period. Despite the degree of particle stability, prostate cells intracellularly received nanoparticles from each of the three nanoparticle systems. Cellular encapsulation of iron nanoparticles by prostate cells was demonstrated with TEM. Iron filing and cell mobility in response to a magnet was captured with video. The gum arabic nanoparticle system exhibited the highest differential uptake (ratio 6.8) at a lower nanoparticle concentration (2.5 x 10 16). There is possibility of greater specificity when the size of prostate cancer and normal cells are compared.
机译：通过共沉淀法从Fe2 +和Fe3 +合成磁性纳米颗粒（MNP）。两个纳米颗粒表面涂层（阿拉伯树胶和柠檬酸钠）提供了额外的功能化和细胞选择性。将表面改性剂掺入到合成的纳米颗粒中产生了基于该研究的三个纳米颗粒系统。物理和化学性质用多种标准表征技术测定。在表征纳米颗粒后，进行了体外细胞培养实验。使纳米颗粒在无菌条件下与前列腺细胞共存48小时。研究了一系列氧化铁纳米颗粒浓度（0.5 x 1016-50 x 1016颗粒/ mL），并比较了它们对细胞生存力，细胞间摄取和前列腺细胞选择性的定量分析的影响;纳米颗粒具有自然凝聚的趋势;因此，添加阿拉伯树胶作为稳定剂可增加静电排斥力，使颗粒自由分散，并极大地促进了水和细胞营养培养基中颗粒的稳定性。柠檬酸钠是水中纳米粒子的极佳封端剂，但在富含血清的营养培养基中却不是。介质的含量导致纳米颗粒溶液的不稳定。因此，随着时间的流逝，介质中柠檬酸钠涂覆的颗粒以及介质中未涂覆的纳米颗粒的粒径测量值会随着时间的推移而降低，形成较大的颗粒（350-375 nm），然后逐渐从溶液中掉出来。与水中稳定的柠檬酸钠颗粒（160 nm）相比，培养基中柠檬酸钠MNP的初始测量值高四倍以上。未涂覆的纳米颗粒表现出在水中的团聚，并且在48小时内尺寸测量值增加到700nm。尽管颗粒具有一定程度的稳定性，但是前列腺细胞在细胞内从三个纳米颗粒系统中的每一个接收纳米颗粒。透射电镜证明了前列腺细胞对铁纳米颗粒的细胞包封。视频记录了铁屑和细胞对磁铁的反应性。阿拉伯树胶纳米颗粒系统在较低的纳米颗粒浓度（2.5 x 10 16）下表现出最高的差异摄取（比率6.8）。当比较前列腺癌和正常细胞的大小时，可能会有更大的特异性。

著录项

  • 作者

    Jackson, Nefertiti Patrick.;

  • 作者单位

    University of Michigan.;

  • 授予单位 University of Michigan.;
  • 学科 Biology Cell.;Health Sciences Oncology.;Biophysics General.;Engineering Biomedical.
  • 学位 Ph.D.
  • 年度 2011
  • 页码 122 p.
  • 总页数 122
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号