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首页> 外文期刊>Neoplasma: Journal of Experimental and Clinical Oncology >The intensity of internalization and cytotoxicity of superparamagnetic iron oxide nanoparticles with different surface modifications in human tumor and diploid lung cells.
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The intensity of internalization and cytotoxicity of superparamagnetic iron oxide nanoparticles with different surface modifications in human tumor and diploid lung cells.

机译：具有不同表面修饰的超顺磁性氧化铁纳米粒子在人肿瘤和二倍体肺细胞中的内在化强度和细胞毒性。

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摘要

The human lung adenocarcinoma epithelial (A549) cells and the human embryo lung (HEL 12469) cells were used to investigate the uptake and cytotoxicity of magnetite nanoparticles (MNPs) with different chemically modified surfaces. MNPs uptake was an energy-dependent process substantially affected by the serum concentration in the culture medium. Internalized MNPs localized in vesicle-bound aggregates were observed in the cytoplasm, none in the nucleus or in mitochondria. All MNPs induced a?dose- and time-dependent increase in cytotoxicity in both human lung cell lines. The cytotoxicity of MNPs increased proportionally with the particle size. Since the cytotoxicity of MNPs was nearly identical when the doses were equalized based on particle surface area, we suppose that the particle surface area rather than the surface modifications per se underlay the cytotoxicity of MNPs. In general, higher internalized amount of MNPs was found in HEL 12469 cells compared with A549 cells. Accordingly, the viability of the human embryo lung cells was reduced more substantially than that of the adenocarcinoma lung cells. The weak MNPs uptake into A549 cells might be of biomedical relevance in cases where MNPs should be used as nanocarriers for targeted drug delivery in tumor tissue derived from alveolar epithelial cells. Keywords: magnetite nanoparticles, surface modifications, cellular uptake, cytotoxicity.
机译：使用人类肺腺癌上皮（A549）细胞和人类胚胎肺（HEL 12469）细胞来研究具有不同化学修饰表面的磁铁矿纳米颗粒（MNP）的摄取和细胞毒性。 MNP的摄取是一个能量依赖的过程，受培养基中血清浓度的影响很大。在细胞质中未观察到位于囊泡结合的聚集物中的内在化的MNP，在细胞核或线粒体中均未观察到。所有的MNPs都在两个人肺细胞系中诱导剂量和时间依赖性的细胞毒性增加。 MNP的细胞毒性与粒径成正比。由于当基于颗粒表面积使剂量相等时，MNP的细胞毒性几乎相同，因此我们认为颗粒表面积而非表面修饰本身本身就是MNP的细胞毒性的基础。通常，与A549细胞相比，在HEL 12469细胞中发现了更高的MNP内部化量。因此，人胚肺细胞的生存能力比腺癌肺细胞的生存能力大大降低。在应将MNP用作纳米载体以在源自肺泡上皮细胞的肿瘤组织中靶向药物递送的情况下，弱吸收到A549细胞中可能具有生物医学意义。关键词：磁铁矿纳米粒子，表面改性，细胞吸收，细胞毒性。

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