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首页> 外文期刊>NanoBioscience, IEEE Transactions on >Fabrication and Characterization of Silk-Fibroin-Coated Quantum Dots
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Fabrication and Characterization of Silk-Fibroin-Coated Quantum Dots

机译:丝素蛋白涂层量子点的制备与表征

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We report a novel technique of directly coating colloidal CdSe/ZnS core/shell quantum dots (QDs) with silk fibroin (SF), a protein derived from the Bombyx mori silk worm. The approach results in protein-modified QDs with little or no particle aggregation, and mitigates the issue of biocompatibility. QDs have desirable optical properties, such as narrow-band emission, broadband absorption, high quantum yield, and high resistance to photobleaching. SF is a fibrous protein polymer with a biomimetic peptide sequence, water and oxygen permeability, low inflammatory response, no thrombogenecity, and cellular biocompatibility, which are desirable properties for in vivo delivery. Combining the unique properties of QDs with the biocompatibility profile of SF, the approach produces particles representing a powerful tool for numerous in vivo and in vitro applications. The design and preparation of these protein-modified QDs conjugates is reported along with functional characterization using luminescence, transmission electron microscope (TEM), and atomic force microscope (AFM). Additionally, we report results obtained using the QDs conjugates as a fluorescent label for bioimaging HEYA8 ovarian cancer cells.
机译:我们报告了一种直接技术,用蚕丝蛋白(SF)(一种来自家蚕的蚕蛋白)直接包被胶体CdSe / ZnS核/壳量子点(QDs)的新技术。该方法导致蛋白质修饰的量子点几乎没有或没有粒子聚集,并减轻了生物相容性问题。 QD具有理想的光学特性,例如窄带发射,宽带吸收,高量子产率和高抗光漂白性。 SF是一种纤维蛋白聚合物,具有仿生肽序列,水和氧渗透性,低炎症反应,无血栓形成性和细胞生物相容性,是体内递送的理想特性。将QD的独特特性与SF的生物相容性相结合,该方法产生的颗粒代表了许多体内和体外应用的有力工具。这些蛋白质修饰的QDs共轭物的设计和制备以及使用发光,透射电子显微镜(TEM)和原子力显微镜(AFM)的功能表征都得到了报道。此外,我们报告了使用QDs偶联物作为生物成像HEYA8卵巢癌细胞的荧光标记获得的结果。

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