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首页> 外文期刊>NanoBioscience, IEEE Transactions on >Differences in DNA Damage Pathways Induced by Two Ceramic Nanoparticles
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Differences in DNA Damage Pathways Induced by Two Ceramic Nanoparticles

机译：两种陶瓷纳米粒子诱导的DNA损伤途径的差异

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In our prophase studies, it has been proved that hydroxyapatite (HAP) and tricalcium phosphate (TCP) nanoparticles (NPs) had obvious cytotoxicity on rat macrophages. So, mechanisms of DNA damage induced by HAP and TCP NPs would be discussed in these studies. Rat peritoneal macrophages were cultured and induced by NPs in vitro. Then, the expressions of P53, P21, growth arrest and DNA damage 45 (Gadd45), and heat shock protein 70 (HSP70) were examined by reverse transcription polymerase chain reaction. The results showed that the expressions of P53, P21, and HSP70 increased with increasing concentrations of HAP NPs. The expressions of P53 and HSP70 were clearly higher than the negative control at 100 mug/mL HAP NPs (p 0.05). Furthermore, 20 mug/mL TCP NPs could markedly induce the expression of all four genes (p < 0.05), although their expression decreased with increasing concentration of TCP NPs. These studies confirm that only 20 mug/mL TCP NPs could induce DNA damage compared with 100 mug/mL HAP NPs. HAP NPs induced cell cycle arrest to allow enough time for DNA repair, while TCP NPs simultaneously promoted the removal of damaged nucleotides and cell cycle arrest to repair the damaged DNA. DNA damage was irreversible when the concentration of these NPs was greater than 200 mu g/mL. Therefore, HAP and TCP NPs induce DNA damage at the molecular level and induce different DNA damage responses.
机译：在我们的前期研究中，已证明羟基磷灰石（HAP）和磷酸三钙（TCP）纳米颗粒（NPs）对大鼠巨噬细胞具有明显的细胞毒性。因此，将在这些研究中讨论由HAP和TCP NP引起的DNA损伤的机制。大鼠腹膜巨噬细胞的体外培养和NPs诱导。然后，通过逆转录聚合酶链反应检查P53，P21，生长停滞和DNA损伤45（Gadd45）和热休克蛋白70（HSP70）的表达。结果表明，随着HAP NP浓度的增加，P53，P21和HSP70的表达增加。在100杯/毫升HAP NPs下，P53和HSP70的表达明显高于阴性对照（p 0.05）。此外，尽管20个马克杯/毫升的TCP NPs的表达随TCP NPs浓度的增加而降低，但可以显着诱导所有四个基因的表达（p <0.05）。这些研究证实，与100杯/ mL HAP NP相比，仅20杯/ mL TCP NP可以诱导DNA损伤。 HAP NPs诱导细胞周期停滞，以便有足够的时间进行DNA修复，而TCP NPs同时促进了受损核苷酸的去除和细胞周期停滞以修复受损的DNA。当这些NP的浓度大于200μg/ mL时，DNA损伤是不可逆的。因此，HAP和TCP NP在分子水平上诱导DNA损伤并诱导不同的DNA损伤反应。

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