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首页> 外文期刊>Nanobiotechnology, IET >Amino-functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5-furandicarboxylic acid
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Amino-functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5-furandicarboxylic acid

机译：氨基官能化的介孔二氧化硅微球，用于固定念珠菌抗原脂肪酶B - 适用于2,5-呋喃羧酸的更环保

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摘要

In the present study, amino-functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2,5-furandicarboxylic acid (FDCA) from 2,5-diformylfuran (DFF). Under the optimised operating conditions of pH 6.5, particle/enzyme ratio of 1.25:1.0 and glutaraldehyde concentration of 4 mM, a maximum CaLB immobilisation yield of 82.4% on silica microspheres was obtained in 12.25 h. The immobilised CaLB was used for the synthesis of alkyl esters, which were utilised along with hydrogen peroxide for FDCA synthesis. The biocatalytic conversion of 30 mM DFF dictated a 77-79% FDCA in 48 h at 30 degrees C; where the turnover number and turnover frequency of immobilised CaLB were 6220.73 mol mol(-1) and 129.59 h(-1), respectively, for ethyl acetate, against 6297.65 mol mol(-1) and 131.2 h(-1), respectively, for ethyl butyrate. Upon examining the operational stability, the immobilised CaLB exhibited high stability till five cycles of FDCA production.
机译：在本研究中，氨基官能化的介孔二氧化硅微球用作Candida抗野胶脂肪酶B（CALB）的共价固定，从2,5-二甲基呋喃（DFF）中产生2,5-呋喃二羧酸（FDCA） 。在pH 6.5的优化操作条件下，粒子/酶比为1.25：1.0和戊二醛浓度为4mm，在12.25小时内获得了二氧化硅微球的最大CALB固定收率82.4％。固定的CALB用于合成烷基酯，其与过氧化氢一起使用以进行FDCA合成。 30 mm DFF的生物催化转化在30摄氏度的48小时内决定了77-79％的FDCA;如果固定的钙的数量和周转频率分别为6220.73mol（-1）和129.59h（-1），分别用于6297.65mol（-1）和131.2h（-1），对于丁酸乙酯。在检查操作稳定性后，固定的CALB表现出高稳定性直至5个FDCA生产循环。

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