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Role of catalytic protein and stabilising agents in the transformation of Ag ions to nanoparticles by Pseudomonas aeruginosa

机译：铜绿假单胞菌中催化蛋白和稳定剂在银离子向纳米颗粒转化中的作用

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摘要

Biological routes of synthesising metal nanoparticles (NPs) using microbes have been gaining much attention due to their low toxicity and eco-friendly nature. Pseudomonas aeruginosa JP2 isolated from metal contaminated soil was evaluated towards extracellular synthesis of silver NPs (AgNPs). Cell-free extract (24 h) of the bacterial isolate was reacted with AgNO3 for 24 h in order to fabricate AgNPs. Preliminary observations were recorded in terms of colour change of the reaction mixture from yellow to greyish black. UV-visible spectroscopy of the reaction mixture has shown a progressive increase in optical densities that correspond to peaks near 430 nm, depicting reduction of ionic silver (Ag+) to atomic silver (Ag0) thereby synthesising NPs. X-ray diffraction spectra exhibited the 2θ values to be 38.4577° confirming the crystalline and spherical nature of NPs [9.6 - 26.7 (Ave. = 17.2 nm)]. Transmission electron microscopy finally confirmed the size of the particles varying from 5 to 60 nm. Moreover, rhamnolipids and proteins were identified as stabilising molecules for the AgNPs through Fourier transform-infrared spectroscopy. Characterisation of bacterial crude and purified protein fractions confirmed the involvement of nitrate reductase (molecular weight 66 kDa and specific activity = 3.8 U/mg) in the Synthesis of AgNPs.
机译：由于微生物的低毒性和生态友好性，使用微生物合成金属纳米颗粒（NPs）的生物途径已引起广泛关注。从金属污染土壤中分离出的铜绿假单胞菌JP2被评估用于银NPs（AgNPs）的细胞外合成。使细菌分离物的无细胞提取物（24小时）与AgNO3反应24小时，以制备AgNP。记录了从反应混合物从黄色到灰黑色的颜色变化的初步观察结果。反应混合物的紫外-可见光谱显示出对应于430 nm附近峰的光密度逐渐增加，表明离子银（Ag +）还原为原子银（Ag0），从而合成了NPs。 X射线衍射光谱显示2θ值为38.4577°，确认了NP的晶体和球形性质[9.6-26.7（Ave. = 17.2 nm）]。透射电子显微镜最终证实了颗粒的大小在5至60 nm之间变化。此外，鼠李糖脂和蛋白质通过傅立叶变换红外光谱法被鉴定为AgNPs的稳定分子。细菌粗蛋白和纯化蛋白组分的鉴定证实了硝酸还原酶（分子量66 kDa，比活性= 3.8 U / mg）参与了AgNP的合成。

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