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首页> 外文期刊>Nanobiotechnology, IET >Elucidation of biogenic silver nanoparticles susceptibility towards Escherichia coli: an investigation on the antimicrobial mechanism
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Elucidation of biogenic silver nanoparticles susceptibility towards Escherichia coli: an investigation on the antimicrobial mechanism

机译：阐明生物型银纳米颗粒对大肠杆菌的敏感性：抗菌机制的研究

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摘要

Elucidation of the role of silver nanoparticles (AgNPs) in combating bacterial infection is important for the development of new antimicrobial compounds. In this study, several key factors underlying biological effects of biogenic AgNPs were investigated on recombinant Escherichia coli (XL1-Blue) which contains a reporter gene encoding β-galactosidase enzyme. Biogenic AgNPs were prepared from the tea decoction. Cytotoxicity effects were profound on the bacteria tested by the synthesised NPs. The β-galactosidase activity of the released intracellular proteins in the supernatant of E. coli was used as a measure of membrane damage and cellular leakage. Occurrence of a significant amount of β-galactosiadase activity in the supernatant of treated cells clearly demonstrated the formation of holes in the bacterial membrane. Scanning electron microscope pictures visibly indicated destruction of the membrane of the bacteria, which further confirmed membrane damage. The synthesised NPs caused damage of E. coli genomic DNA in a dose dependent manner.
机译：阐明银纳米颗粒（AgNPs）在抵抗细菌感染中的作用对于开发新的抗菌化合物很重要。在这项研究中，研究了生物AgNP的生物学效应的几个关键因素对重组大肠杆菌（XL1-Blue）的影响，该大肠杆菌包含一个编码β-半乳糖苷酶的报告基因。由茶汤制备生物AgNP。细胞毒性对合成的NP测试的细菌有深远的影响。大肠杆菌上清液中释放的细胞内蛋白的β-半乳糖苷酶活性用作膜损伤和细胞渗漏的量度。在处理过的细胞的上清液中出现大量的β-半乳糖苷酶活性，清楚地证明了细菌膜上形成了孔。扫描电子显微镜图像可见地表明了细菌膜的破坏，这进一步证实了膜的破坏。合成的NP以剂量依赖性方式引起大肠杆菌基因组DNA的损伤。

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