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Nitrobacter sp. extract mediated biosynthesis of Ag2O NPs with excellent antioxidant and antibacterial potential for biomedical application

机译:硝化细菌提取物介导的Ag2O NPs的生物合成,具有极好的抗氧化和抗菌潜力,可用于生物医学应用

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摘要

In this study, extracellular extract of plant growth promoting bacterium, Nitrobacter sp. is used for the bioconversion of AgNO3 (silver nitrate) into Ag2O (silver oxide nanoparticles). It is an easy, ecofriendly and single step method for Ag2O NPs synthesis. The bio-synthesized nanoparticles were characterized using different techniques. UV-Vis results showed the maximum absorbance around 450 nm. XRD result shows the particles to have faced centered cubic (fcc) crystalline nature. FTIR analysis reveals the functional groups that are involved in bioconversion such as C–N, N–H and C=O. Energy-dispersive X-ray spectroscopy (EDAX) spectrum confirms that the prepared nanoparticle is Ag2O NPs. Particle size distribution result reveals that the average particle size is around 40 nm. The synthesized Ag2O NPs found to be almost spherical in shape. Biosynthesized Ag2O NPs possess good antibacterial activity against selected Gram positive and Gram negative bacterial strains namely Salmonella typhimurium, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae when compared to standard antibiotic. In addition, Ag2O NPs exhibits excellent free radical scavenging activity with respect to dosage. Thus, this study is a new approach to use soil bacterial extract for the production of Ag2O NPs for biomedical application.
机译:在这项研究中,植物生长促进细菌硝化细菌菌种的细胞外提取物。用于将AgNO3(硝酸银)生物转化为Ag2O(氧化银纳米粒子)。这是一种简单,环保且一步一步的Ag2O NP合成方法。使用不同技术对生物合成的纳米颗粒进行了表征。 UV-Vis结果显示最大吸收在450 nm附近。 XRD结果显示颗粒具有面对中心的立方(fcc)晶体性质。 FTIR分析揭示了与生物转化有关的官能团,例如C–N,NH–和C = O。能量色散X射线光谱(EDAX)光谱证实所制备的纳米颗粒为Ag 2 O NPs。粒径分布结果表明,平均粒径为40nm左右。发现合成的Ag 2 O NPs几乎为球形。与标准抗生素相比,生物合成的Ag2O NPs对选定的革兰氏阳性和革兰氏阴性细菌菌株鼠伤寒沙门氏菌,金黄色葡萄球菌,大肠杆菌和肺炎克雷伯菌具有良好的抗菌活性。另外,相对于剂量,Ag 2 O NPs表现出优异的自由基清除活性。因此,这项研究是使用土壤细菌提取物生产用于生物医学应用的Ag2O NP的新方法。

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