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DNA adduct identification using gold-aptamer nanoprobes

机译：使用金适体纳米探针鉴定DNA加合物

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摘要

The optical and physico-chemical properties of gold nanoparticles (AuNPs) have prompted new and improved approaches which have greatly evolved the fields of biosensing and molecular detection. In this study, the authors took advantage of AuNPs' ease of modification and functionalised it with selected DNA aptamers using a salt aging method to produce gold-aptamer nanoprobes. After characterisation, these nanoprobes were subsequently used for biomolecular detection of glycidamide (GA)-guanine (Gua) adducts generated . The results are based on differences in nanoprobe stabilisation against salt-induced aggregation, similar to the non-cross-linking method developed by Baptista for discrimination of specific sequences. Alkylated Guas were efficiently discriminated from deoxyguanosine and GA in solution. Despite this, a clear identification of DNA adducts derived from genomic DNA alkylation has proven to be a more challenging task.
机译：金纳米粒子（AuNPs）的光学和物理化学性质促使人们提出了新的改进方法，这些方法极大地发展了生物传感和分子检测领域。在这项研究中，作者利用了AuNPs的易修饰性，并使用盐老化法将其与选定的DNA适体一起功能化，从而制备了金适体纳米探针。表征后，这些纳米探针随后用于产生的缩水甘油酰胺（GA）-鸟嘌呤（Gua）加合物的生物分子检测。该结果基于针对盐诱导的聚集的纳米探针稳定性的差异，类似于Baptista为区分特定序列而开发的非交联方法。有效地将烷基化的瓜豆与溶液中的脱氧鸟苷和GA区分开。尽管如此，从基因组DNA烷基化中明确鉴定DNA加合物已被证明是一项更具挑战性的任务。

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