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首页> 外文期刊>Nanobiotechnology, IET >Comparative analysis of biosynthesised and chemosynthesised silver nanoparticles with special reference to their antibacterial activity against pathogens
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Comparative analysis of biosynthesised and chemosynthesised silver nanoparticles with special reference to their antibacterial activity against pathogens

机译：生物合成和化学合成的银纳米颗粒的比较分析，特别是它们对病原体的抗菌活性

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摘要

The present study reports the synthesis of silver nanoparticles (AgNPs) using both biological and chemical routes to find out the best method for control of their size and activity. The fungal agent ( and the plant ( were found to be the best source for AgNPs synthesis. Both biosynthesis and chemosynthesis were achieved by challenging filtrate with AgNO (1 mM) solution. The synthesised nanoparticles were characterised by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, nanoparticle tracking analysis (LM20), zeta potential measurement and transmission electron microscopy. The biologically synthesised nanoparticles were spherical, polydispersed and in the range of 10-40 nm, while chemically synthesised nanoparticles were highly monodispersed with a size of 5 nm. The antimicrobial assay against and proved biogenic AgNPs to be more potent antibacterial agents than chemically synthesised AgNPs. The possible antibacterial mechanism of AgNPs has also been discussed. Biogenic AgNPs have shown more activity because of the protein capping and their mode of entry into the bacterial cell. These findings may encourage the use of biosynthesis over the chemosynthesis method.
机译：本研究报告了使用生物学和化学途径合成银纳米颗粒（AgNPs）的方法，以找到控制其大小和活性的最佳方法。已发现真菌剂（和植物）是合成AgNPs的最佳来源。通过用AgNO（1 mM）溶液挑战滤液可实现生物合成和化学合成。通过紫外可见光谱，傅里叶变换红外光谱对合成的纳米颗粒进行表征。光谱学，纳米粒子跟踪分析（LM20），ζ电势测量和透射电子显微镜观察法，生物合成的纳米粒子呈球形，多分散且在10-40 nm的范围内，而化学合成的纳米粒子则高度单分散，尺寸为5 nm。抗菌测定法证明了生物合成的AgNPs比化学合成的AgNPs更有效，还讨论了AgNPs可能的抗菌机理，由于蛋白质的封端及其进入细菌细胞的方式，生物AgNPs表现出更大的活性。这些发现可能会鼓励生物合成的应用。化学合成方法。

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