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首页> 外文期刊>Nanobiotechnology, IET >Plumbagin-silver nanoparticle formulations enhance the cellular uptake of plumbagin and its antiproliferative activities
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Plumbagin-silver nanoparticle formulations enhance the cellular uptake of plumbagin and its antiproliferative activities

机译：Plumbagin-银纳米颗粒制剂可增强Plumbagin的细胞摄取及其抗增殖活性

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摘要

Colloidal silver nanoparticles (AgNPs) have attracted much attention in recent years as diagnostics and new drug delivery system in cancer medicine. To study the effects of plumbagin (PLB), a relatively non-toxic napthaquinone isolated from the roots of in human cervical cancer cell line and developed a formulation to enhance its cytotoxic activities. Silver nanoparticles were synthesised by chemical reduction method and complexed with PLB. Both the AgNPs and the complex PLB-AgNPs were characterised by dynamic light scattering, high-resolution scanning electron microscopy and transmission electron microscopy. The amount of PLB and PLB-AgNPs internalised was determined by ultra-violet-visible spectrophotometer. Cell inhibition was determined by sulphorhodamine B assay. Mitotic index was determined by Wright-Giemsa staining. Apoptosis induction was assessed by western blot using cleaved poly adenosine diphosphate-ribose polymerase antibody. The scanning electron microscope analysis indicated an average particle size of 32 ± 8 nm in diameter. Enhanced internalisation of PLB into the HeLa cells was observed in PLB-AgNPs. PLB inhibited proliferation of cells with IC value of about 18 ± 0.6 μM and blocked the cells at mitosis in a concentration-dependent manner. PLB also inhibited the post-drug exposure clonogenic survival of cells and induced apoptosis. The antiproliferative, antimitotic and apoptotic activities were also found to be increased when cells were treated with PLB-AgNPs. The authors results support the idea that AgNP could be a promising and effective drug delivery system for enhanced activity of PLB in cancer treatment.
机译：近年来，胶体银纳米颗粒（AgNPs）作为癌症医学中的诊断方法和新药物递送系统受到了广泛关注。为了研究plumbagin（PLB）的作用，plumbagin是一种相对无毒的萘醌，它是从人宫颈癌细胞系的根中分离出来的，并开发了一种增强其细胞毒性活性的制剂。通过化学还原法合成银纳米颗粒，并与PLB络合。 AgNPs和复杂的PLB-AgNPs都通过动态光散射，高分辨率扫描电子显微镜和透射电子显微镜来表征。用紫外可见分光光度计测定内在的PLB和PLB-AgNPs的量。细胞抑制通过磺基罗丹明B测定法测定。通过Wright-Giemsa染色确定有丝分裂指数。使用裂解的聚腺苷二磷酸-核糖聚合酶抗体通过蛋白质印迹评估凋亡诱导。扫描电子显微镜分析表明直径平均粒径为32±8nm。在PLB-AgNPs中观察到PLB增强进入HeLa细胞的内部化。 PLB抑制细胞增殖，IC值约为18±0.6μM，并以浓度依赖的方式阻断有丝分裂的细胞。 PLB还抑制药物暴露后细胞的克隆形成存活并诱导凋亡。当用PLB-AgNPs处理细胞时，还发现抗增殖，抗有丝分裂和凋亡活性增加。作者的结果支持这样的想法，即AgNP可能是一种有前途且有效的药物递送系统，可增强PLB在癌症治疗中的活性。

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