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Predicting the fluorescent enhancement rate by gold and silver nanospheres using finite-difference time-domain analysis

机译:使用时域有限差分法预测金和银纳米球的荧光增强速率

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摘要

Metal-induced fluorescence enhancement (MIFE) is a promising strategy for increasing the sensitivity of fluorophores used in biological sensors. This study uses the finite-difference time-domain technique to predict the fluorescent enhancement rate of a fluorophore molecule in close proximity to a gold or silver spherical nanoparticle. By considering commercially available fluorescent dyes the computed results are compared with the published experimental data. The results show that MIFE is a complex coupling process between the fluorophore molecule and the metal nanoparticle. Nevertheless using computational electromagnetic techniques to perform calculations it is possible to calculate, with reasonable accuracy, the fluorescent enhancement. Using this methodology it will be possible to consider different shaped metal nanoparticles and any supporting substrate material in the future, an important step in building reliable biosensors capable of detecting low levels of proteins tagged with fluorescence molecules.
机译:金属诱导的荧光增强(MIFE)是提高生物传感器中使用的荧光团灵敏度的一种有前途的策略。这项研究使用有限差分时域技术来预测紧邻金或银球形纳米粒子的荧光团分子的荧光增强速率。通过考虑可商购的荧光染料,将计算结果与已发布的实验数据进行比较。结果表明,MIFE是荧光团分子与金属纳米粒子之间的复杂偶联过程。然而,使用计算电磁技术来执行计算,仍可以以合理的精度计算荧光增强。使用这种方法,将来有可能考虑使用不同形状的金属纳米颗粒和任何支持的基材材料,这是构建可靠的生物传感器的重要步骤,该传感器能够检测带有荧光分子的低水平蛋白质。

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