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首页> 外文期刊>Nature environment and pollution technology >Identification and Characterization of the Effectively Arsenic Tolerant Bacterial Strains from the Potential Arsenic Contaminated site in 24-Parganas (North) District of West Bengal, India
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Identification and Characterization of the Effectively Arsenic Tolerant Bacterial Strains from the Potential Arsenic Contaminated site in 24-Parganas (North) District of West Bengal, India

机译：从印度西孟加拉邦24-Parganas（北）区的潜在砷污染位点中有效耐受砷的细菌菌株的鉴定和表征

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摘要

Arsenic is a common menace in the state of West Bengal particularly in the district of 24-Parganas (North) and (South). The major source of arsenic being geologic in nature. Soil samples collected from one such particular location Baduria were tested for the presence of arsenic (As), and on an average the As content of the soil was 2.18mg/kg. Different bacteria were isolated by serial dilution and were also tested for As tolerance. On the basis of their tolerance, three organisms (viz. C-7,A-2,A-1) have shown remarkable percentage uptake of As 43%, 22.17% and 14% respectively, which was highest for C-7 strain. Azithromycin and Streptomycin were found to be more effective to control C-7 with an inhibition zone of 4.5 cm, but for A-2 and A-1 it was Azithromycin with inhibition zone of 3.8cm and 3.5cm respectively. These organisms were characterized and identified by 16SrDNAtechnique, BLAST results and the cluster analysis by NTSYSpc ver. 2.02e program, and found to be Bacillus sp. strain CCBAU 51490 (C-7) (GenBankAccession Number EF377314.1), Bacillus subtilis sub sp. subtilis strain DSM 10 (Genbank Accession Number NR_027552.1) (A-2) and Pseudomonas otidis strain MCC 10330 (Genbank Accession Number NR_043289.1) (A-1). They can be used in the treatment of As contaminated sludge generated industrially from As treatment plant. This can give a new insight in microbial arsenic trapping with minimum toxic implications in the nature.
机译：砷是西孟加拉邦常见的威胁，特别是在24-帕加纳斯州（北部）和（南部）地区。砷的主要来源是地质性质。测试了从这样一个特定地点Baduria收集的土壤样品中砷（As）的存在，平均土壤中As含量为2.18mg / kg。通过连续稀释分离不同的细菌，并且还测试其As耐受性。基于它们的耐受性，三种生物（即C-7，A-2，A-1）分别显示出显着的As吸收百分比，分别为43％，22.17％和14％，这对于C-7菌株最高。发现阿奇霉素和链霉素对C-7的抑制范围为4.5 cm，更有效，但对于A-2和A-1，阿奇霉素的抑制范围分别为3.8cm和3.5cm。通过16SrDNA技术，BLAST结果和NTSYSpc ver。 2.02e程序，发现是芽孢杆菌。菌株CCBAU 51490（C-7）（GenBank登录号EF377314.1），枯草芽孢杆菌亚种枯草芽孢杆菌菌株DSM 10（Genbank登录号NR_027552.1）（A-2）和假单孢假单胞菌MCC 10330（Genbank登录号NR_043289.1）（A-1）。它们可用于处理由As处理厂工业产生的As污染污泥。这可以为微生物砷捕集提供新的见解，而对自然界的毒性最小。

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