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Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells

机译:细胞分裂素信号的弥漫性抑制产生内胚层对称性和传代细胞

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摘要

In vascular plants, the root endodermis surrounds the central vasculature as a protective sheath that is analogous to the polarized epithelium in animals, and contains ring-shaped Casparian strips that restrict diffusion(1). After an initial lag phase, individual endodermal cells suberize in an apparently random fashion to produce 'patchy' suberization that eventually generates a zone of continuous suberin deposition(2). Casparian strips and suberin lamellae affect paracellular and transcellular transport, respectively. Most angiosperms maintain some isolated cells in an unsuberized state(3) as so-called 'passage cells', which have previously been suggested to enable uptake across an otherwise impermeable endodermal barrier(3,4). Here we demonstrate that these passage cells are late emanations of a meristematic patterning process that reads out the underlying non-radial symmetry of the vasculature. This process is mediated by the non-cell-autonomous repression of cytokinin signalling in the root meristem, and leads to distinct phloem- and xylem-pole-associated endodermal cells. The latter cells can resist abscisic acid-dependent suberization to produce passage cells. Our data further demonstrate that, during meristematic patterning, xylem-pole-associated endodermal cells can dynamically alter passage-cell numbers in response to nutrient status, and that passage cells express transporters and locally affect the expression of transporters in adjacent cortical cells.
机译:在维管植物中,根皮层围绕着中央脉管系统作为保护鞘,类似于动物中的极化上皮,并包含限制扩散的环形Casparian条(1)。在最初的滞后阶段之后,各个内胚层细胞以明显随机的方式进行干化,以产生“斑片状”的干化作用,最终产生连续的木栓质沉积区域(2)。里海带和木栓质薄片分别影响细胞旁和跨细胞的运输。大多数被子植物将一些分离的细胞维持在未充液状态(3),即所谓的“传代细胞”,这些传代细胞以前曾被建议能够穿过原本不可渗透的内胚层屏障吸收(3,4)。在这里,我们证明这些传代细胞是分生组织构图过程的后期发散,该过程读​​出了脉管系统的潜在非径向对称性。该过程由根分生组织中细胞分裂素信号传导的非细胞自主抑制介导,并导致与韧皮部和木质部极相关的内胚层细胞不同。后者的细胞可以抵抗脱落酸依赖性的枯草化作用以产生传代细胞。我们的数据进一步证明,在分生组织构图过程中,木质部相关的内胚层细胞可以根据营养状况动态改变传代细胞数,并且传代细胞表达转运蛋白,并局部影响邻近皮质细胞中转运蛋白的表达。

著录项

  • 来源
    《Nature》 |2018年第7697期|529-533|共5页
  • 作者单位

    Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland;

    Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland;

    Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland;

    Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium;

    Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium;

    Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium;

    Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland;

    Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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