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首页> 外文期刊>Nature >mRNA 3 ' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome
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mRNA 3 ' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome

机译：mRNA 3'尿苷化和poly（A）尾巴长度雕刻了哺乳动物母本转录组

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摘要

A fundamental principle in biology is that the program for early development is established during oogenesis in the form of the maternal transcriptome(1,2). How the maternal transcriptome acquires the appropriate content and dosage of transcripts is not fully understood. Here we show that 3' terminal uridylation of mRNA mediated by TUT4 and TUT7 sculpts the mouse maternal transcriptome by eliminating transcripts during oocyte growth. Uridylation mediated by TUT4 and TUT7 is essential for both oocyte maturation and fertility. In comparison to somatic cells, the oocyte transcriptome has a shorter poly(A) tail and a higher relative proportion of terminal oligo-uridylation. Deletion of TUT4 and TUT7 leads to the accumulation of a cohort of transcripts with a high frequency of very short poly(A) tails, and a loss of 3' oligouridylation. By contrast, deficiency of TUT4 and TUT7 does not alter gene expression in a variety of somatic cells. In summary, we show that poly(A) tail length and 3' terminal uridylation have essential and specific functions in shaping a functional maternal transcriptome.
机译：生物学的基本原理是在卵子形成过程中以母体转录组的形式建立早期发育程序（1,2）。母体转录组如何获得适当的转录物含量和剂量的方法尚未完全了解。在这里，我们显示TUT4和TUT7介导的mRNA的3'末端尿苷化通过消除卵母细胞生长过程中的转录物来雕刻小鼠母本的转录物。 TUT4和TUT7介导的Uridylation对卵母细胞的成熟和受精都至关重要。与体细胞相比，卵母细胞转录组具有较短的poly（A）尾巴和较高的末端寡尿苷酸化相对比例。 TUT4和TUT7的删除会导致大量的非常短的poly（A）尾巴出现，并丢失3'寡尿酸化作用，从而导致大量的转录物积累。相比之下，TUT4和TUT7的缺乏不会改变多种体细胞中的基因表达。总之，我们表明，poly（A）尾巴长度和3'末端尿苷化在塑造功能性母体转录组中具有必不可少的特定功能。

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  • 来源
    《Nature》 |2017年第7667期|347-351|共5页
  • 作者

    Morgan Marcos; Much Christian; DiGiacomo Monica; Azzi Chiara; Ivanova Ivayla; Vitsios Dimitrios M.; Pistolic Jelena; Collier Paul; Moreira Pedro N.; Benes Vladimir; Enright Anton J.; OCarroll Donal;

  • 作者单位

    Univ Edinburgh, Inst Stem Cell Res, Sch Biol Sci, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland|EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

    Univ Edinburgh, Inst Stem Cell Res, Sch Biol Sci, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland|EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

    EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

    EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

    Univ Edinburgh, Inst Stem Cell Res, Sch Biol Sci, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland|EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

    EMBL, EBI, Wellcome Genome Campus, Cambridge CB10 1SD, England;

    EMBL, Genom Core Facil, D-69117 Heidelberg, Germany;

    EMBL, Genom Core Facil, D-69117 Heidelberg, Germany;

    Univ Edinburgh, Inst Stem Cell Res, Sch Biol Sci, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland|EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

    EMBL, Genom Core Facil, D-69117 Heidelberg, Germany;

    EMBL, EBI, Wellcome Genome Campus, Cambridge CB10 1SD, England;

    Univ Edinburgh, Inst Stem Cell Res, Sch Biol Sci, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland|EMBL, Mouse Biol Unit, I-00015 Monterotondo, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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