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The neural representation of taste quality at the periphery

机译:外围味觉质量的神经表示

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摘要

各种味道(甜味、酸味、苦味、咸味和鲜味)是由舌头和上颚上专门的味觉受体细胞检测的,但这些信号是怎样被编码和传送给中枢神经系统的相关部分(味觉皮层)的却不清楚。Charles Zuker及同事利用在神经元中表达一个钙指示剂的转基因小鼠,表征了神经节神经元(味觉系统的第一个"神经站")的调节特性。神经节神经元只对某些味道有反应,这为关于味觉系统中信息传输的"标线"(labelled line)模型提供了支持。%The mammalian taste system is responsible for sensing and responding to the five basic taste qualities: sweet, sour, bitter, salty and umami. Previously, we showed that each taste is detected by dedicated taste receptor cells (TRCs) on the tongue and palate epithelium. To understand how TRCs transmit information to higher neural centres, we examined the tuning properties of large ensembles of neurons in the first neural station of the gustatory system. Here, we generated and characterized a collection of transgenic mice expressing a genetically encoded calcium indicator in central and peripheral neurons, and used a gradient refractive index microendoscope combined with high-resolution two-photon microscopy to image taste responses from ganglion neurons buried deep at the base of the brain. Our results reveal fine selectivity in the taste preference of ganglion neurons; demonstrate a strong match between TRCs in the tongue and the principal neural afferents relaying taste information to the brain; and expose the highly specific transfer of taste information between taste cells and the central nervous system.
机译:各种味道(甜味、酸味、苦味、咸味和鲜味)是由舌头和上颚上专门的味觉受体细胞检测的,但这些信号是怎样被编码和传送给中枢神经系统的相关部分(味觉皮层)的却不清楚。Charles Zuker及同事利用在神经元中表达一个钙指示剂的转基因小鼠,表征了神经节神经元(味觉系统的第一个"神经站")的调节特性。神经节神经元只对某些味道有反应,这为关于味觉系统中信息传输的"标线"(labelled line)模型提供了支持。%The mammalian taste system is responsible for sensing and responding to the five basic taste qualities: sweet, sour, bitter, salty and umami. Previously, we showed that each taste is detected by dedicated taste receptor cells (TRCs) on the tongue and palate epithelium. To understand how TRCs transmit information to higher neural centres, we examined the tuning properties of large ensembles of neurons in the first neural station of the gustatory system. Here, we generated and characterized a collection of transgenic mice expressing a genetically encoded calcium indicator in central and peripheral neurons, and used a gradient refractive index microendoscope combined with high-resolution two-photon microscopy to image taste responses from ganglion neurons buried deep at the base of the brain. Our results reveal fine selectivity in the taste preference of ganglion neurons; demonstrate a strong match between TRCs in the tongue and the principal neural afferents relaying taste information to the brain; and expose the highly specific transfer of taste information between taste cells and the central nervous system.

著录项

  • 来源
    《Nature》 |2015年第7534期|373-376a3|共5页
  • 作者单位

    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics and of Neuroscience, Columbia College of Physicians and Surgeons, Columbia University, New York 10032, USA;

    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics and of Neuroscience, Columbia College of Physicians and Surgeons, Columbia University, New York 10032, USA;

    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA;

    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics and of Neuroscience, Columbia College of Physicians and Surgeons, Columbia University, New York 10032, USA;

    James H. Clark Center, Stanford University, Stanford, California 94305, USA;

    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA;

    Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biophysics and of Neuroscience, Columbia College of Physicians and Surgeons, Columbia University, New York 10032, USA,Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA;

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