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An Arabidopsis gene regulatory network for secondary cell wall synthesis

机译:用于二次细胞壁合成的拟南芥基因调控网络

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摘要

The plant cell wall is an important factor for determining cell shape, function and response to the environment. Secondary cell walls, such as those found in xylem, are composed of cellulose, hemicelluloses and lignin and account for the bulk of plant biomass. The coordination between transcriptional regulation of synthesis for each polymer is complex and vital to cell function. A regulatory hierarchy of developmental switches has been proposed, although the full complement of regulators remains unknown. Here we present a protein-DNA network between Arabidopsis thaliana transcription factors and secondary cell wall metabolic genes with gene expression regulated by a series of feed-forward loops. This model allowed us to develop and validate new hypotheses about secondary wall gene regulation under abiotic stress. Distinct stresses are able to perturb targeted genes to potentially promote functional adaptation. These interactions will serve as a foundation for understanding the regulation of a complex, integral plant component.%植物细胞壁决定细胞形状,介导与细胞环境的通信。但次生细胞壁(沉积在包括木质部在内的各种细胞类型中)才是用于生物燃料以及制浆和造纸工业的生物质的主要来源。影响次生细胞壁生物合成的全部转录调控因子在很大程度上仍未比确定。Siobhan Brady及同事描述了一个涉及数百个转录因子的基因调控网络,它通过蛋白-DNA相互作用控制拟南芥植物木质部的形成。基因表达是由一系列"前馈环路''调控的,以确保次生细胞壁在正确的时间沉积在正确的地方。作者用他们的基因调控网络来建立关于非生物压力(如盐度和铁剥夺)对次生细胞壁基因调控之影响的新假说并对这些假说进行确认。
机译:植物细胞壁是决定细胞形状,功能和对环境响应的重要因素。次级细胞壁(例如木质部中发现的那些)由纤维素,半纤维素和木质素组成,占植物生物量的大部分。每种聚合物的合成转录调控之间的协调是复杂的,对细胞功能至关重要。尽管尚不清楚调节器的完整补充,但已经提出了开发开关的调节等级。在这里,我们介绍拟南芥转录因子和次级细胞壁代谢基因之间的蛋白质-DNA网络,其基因表达受一系列前馈环调控。该模型使我们能够开发和验证关于非生物胁迫下次生壁基因调控的新假设。不同的压力能够干扰目标基因,从而潜在地促进功能适应。这些相互作用将为理解复杂的整体植物成分的调控奠定基础。%植物细胞壁决定细胞形状,介导与细胞环境的通信。但次生细胞壁(沉积在包括木质部内部的各种细胞类型中)才是用于生物燃料以及制浆和造纸工业的生物质的主要来源。影响次生细胞壁生物合成的全部转化因子在其中仍未比确定。SiobhanBrady及同事描述了一个涉及几个个转录因子的基因分解网络,它通过蛋白-DNA相互作用控制拟南芥植物木质部的形成。基因表达是由一系列“前馈环路”转变的,以确保次生细胞壁在正确的时间沉积在正确的地方。作者用他们的基因分解网络来建立关于非生物压力(如盐度和铁剥夺)对次生细胞壁基因改变之影响的新假说逐渐这些假说进行确认。

著录项

  • 来源
    《Nature》 |2015年第7536期|571-575a2|共6页
  • 作者单位

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA,Division of Plant Sciences, University of Missouri, Columbia, Missouri 65211, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA;

    Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, California 94720, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Department of Computer Science, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA;

    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA,US Department of Agriculture, Agricultural Research Service, Ithaca, New York 14853, USA;

    Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, California 94720, USA;

    Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Department of Computer Science, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA,Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA;

    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA;

    Theory of Condensed Matter Group, Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, UK;

    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA,Molecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089, USA;

    Biology Department, University of Massachusetts, Amherst, Massachusetts 01003, USA;

    Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, California 95616, USA,Genome Center, University of California Davis, One Shields Avenue, Davis, California 95616, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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