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Pyrimidine homeostasis is accomplished by directed overflow metabolism

机译:嘧啶体内稳态是通过定向溢流代谢实现的

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摘要

Cellular metabolism converts available nutrients into usable energy and biomass precursors. The process is regulated to facilitate efficient nutrient use and metabolic homeostasis. Feedback inhibition of the first committed step of a pathway by its final product is a classical means of controlling biosynthesis~(1-4). In a canonical example, the first committed enzyme in the pyrimidine pathway in Escherichia coli is allosterically inhibited by cytidine triphosphate~(1,4,5). The physiological consequences of disrupting this regulation, however, have not been previously explored. Here we identify an alternative regulatory strategy that enables precise control of pyrimidine pathway end-product levels, even in the presence of dysregulated biosyn-thetic flux.
机译:细胞代谢将可用的养分转化为可用的能量和生物质前体。对过程进行调节以促进有效的养分利用和代谢稳态。最终产物对通路的第一个固定步骤的反馈抑制是控制生物合成的一种经典方法[1-4]。在一个典型的例子中,大肠杆菌的嘧啶途径中的第一个定型酶被胞苷三磷酸〜(1,4,5)变构抑制。但是,以前从未研究过破坏这种调节的生理后果。在这里,我们确定了另一种调控策略,即使在生物合成通量失调的情况下,也可以精确控制嘧啶途径终产物的水平。

著录项

  • 来源
    《Nature》 |2013年第7461期|237-241|共5页
  • 作者单位

    Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA,Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA;

    Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA,Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA;

    Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA,Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA;

    Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA,Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA;

    Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA,Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA,Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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