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A map of nucleosome positions in yeast at base-pair resolution

机译：碱基对分辨率下酵母中核小体位置图

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摘要

核小体在基因组DNA上的位置会影响染色体功能的很多方面。Kristin Brogaard等人建立了一种新颖的方法，来在全基因组范围内对酿酒酵母的核小体位置进行活体分析，其办法是利用高度局部化的羟基自由基来修饰工程改造的组蛋白。所获得的分布图以单碱基对的准确性限定了核小体中心的位置。核小体的精确位置被rn发现与各种不同的基因组特征相关，其中包括转位子集成点的精确位置以及RNA聚合酶的停顿点。%The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered his tones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.
机译：核小体在基因组DNA上的位置会影响染色体功能的很多方面。Kristin Brogaard等人建立了一种新颖的方法，来在全基因组范围内对酿酒酵母的核小体位置进行活体分析，其办法是利用高度局部化的羟基自由基来修饰工程改造的组蛋白。所获得的分布图以单碱基对的准确性限定了核小体中心的位置。核小体的精确位置被rn发现与各种不同的基因组特征相关，其中包括转位子集成点的精确位置以及RNA聚合酶的停顿点。%The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered his tones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

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  • 来源
    《Nature》 |2012年第7404期|p.496-501a1|共7页
  • 作者

    Kristin Brogaard; Liqun Xi; Ji-Ping Wang; Jonathan Widom;

  • 作者单位

    Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA;

    Department of Statistics, Northwestern University, Evanston, Illinois 60208, USA;

    Department of Statistics, Northwestern University, Evanston, Illinois 60208, USA;

    Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA, Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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