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Determinants of nucleosome organization in primary human cells

机译:人原代细胞核小体组织的决定因素

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核小体在整个基因组中的组织影响基因调控, 了解不同生物中这种组织的机制是一个活跃的 研究领域。在这项研究中,研究人员通过高吞 吐量的DNA测序方法在体外生成了人的三种主 要细胞类型中核小体位置的全基因组分布图。%Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodelling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome~1. Analyses of chromatin landscape have uncovered a variety of mechanisms, including DNA sequence preferences, that can influence nucleosome positions~(2-4). To identify major determinants of nucleosome organization in the human genome, we used deep sequencing to map nucleosome positions in three primary human cell types and in vitro. A majority of the genome showed substantial flexibility of nucleosome positions, whereas a small fraction showed reproducibly positioned nucleosomes. Certain sites that position in vitro can anchor the formation of nucleosomal arrays that have cell type-specific spacing in vivo. Our results unveil an interplay of sequence-based nucleosome preferences and non-nucleosomal factors in determining nucleosome organization within mammalian cells.
机译:核小体在整个基因组中的组织影响基因调控, 了解不同生物中这种组织的机制是一个活跃的 研究领域。在这项研究中,研究人员通过高吞 吐量的DNA测序方法在体外生成了人的三种主 要细胞类型中核小体位置的全基因组分布图。%Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodelling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome~1. Analyses of chromatin landscape have uncovered a variety of mechanisms, including DNA sequence preferences, that can influence nucleosome positions~(2-4). To identify major determinants of nucleosome organization in the human genome, we used deep sequencing to map nucleosome positions in three primary human cell types and in vitro. A majority of the genome showed substantial flexibility of nucleosome positions, whereas a small fraction showed reproducibly positioned nucleosomes. Certain sites that position in vitro can anchor the formation of nucleosomal arrays that have cell type-specific spacing in vivo. Our results unveil an interplay of sequence-based nucleosome preferences and non-nucleosomal factors in determining nucleosome organization within mammalian cells.

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  • 来源
    《Nature》 |2011年第7352期|p.516-520409|共6页
  • 作者单位

    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA;

    Department of Microbiology and Molecular Biology, Brigham Young University, 757 WIDB, Provo, Utah 84602-5253, USA;

    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA;

    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA;

    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA Department of Genetics, Stanford University School of Medicine, Pasteur Drive, Stanford, California 94305, USA;

    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA Department of Genetics, Stanford University School of Medicine, Pasteur Drive, Stanford, California 94305, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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