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Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis

机译：在光合作用中驱动循环电子流动的难以捉摸的超复合物的分离

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摘要

Photosynthetic light reactions establish electron flow in the chloroplast's thylakoid membranes, leading to the production of the ATP and NADPH that participate in carbon fixation. Two modes of electron flow exist-linear electron flow (LEF) from water to NADP~+ via photosystem (PS) II and PSI in series and cyclic electron flow (CEF) around PSI (ref. 2). Although CEF is essential for satisfying the varying demand for ATP, the exact molecule(s) and operational site are as yet unclear. In the green alga Chlamydomonas reinhardtii, the electron flow shifts from LEF to CEF on preferential excitation of PSII (ref. 3), which is brought about by an energy balancing mechanism between PSII and PSI (state transitions). Here, we isolated a protein super-complex composed of PSI with its own light-harvesting complex (LHCI), the PSII light-harvesting complex (LHCII), the cytochrome b_6f complex (Cyt bf), ferredoxin (Fd)-NADPH oxidoreductase (FNR), and the integral membrane protein PGRL1 (ref. 5) from C. reinhardtii cells under PSII-favouring conditions. Spectro-scopic analyses indicated that on illumination, reducing equivalents from downstream of PSI were transferred to Cyt bf, whereas oxidised PSI was re-reduced by reducing equivalents from Cyt bf, indicating that this supercomplex is engaged in CEF (Supplementary Fig. 1). Thus, formation and dissociation of the PSI-LHCI-LHCII-FNR-Cyt bf-PGRL1 supercomplex not only controlled the energy balance of the two photosystems, but also switched the mode of photosynthetic electron flow.
机译：光合作用的光反应在叶绿体类囊体膜中建立电子流，从而导致参与碳固定的ATP和NADPH的产生。存在两种电子流动模式：从水到光子系统（PS）II和PSI的串联电子从水到NADP〜+的线性电子流（LEF），以及在PSI周围的循环电子流（CEF）（参考文献2）。尽管CEF对于满足不断变化的ATP需求至关重要，但尚不清楚确切的分子和操作位点。在绿藻莱茵衣藻中，在PSII的优先激发下，电子流从LEF转移到CEF（参考文献3），这是由PSII和PSI之间的能量平衡机制引起的（状态转换）。在这里，我们分离了由PSI及其自身的光捕获复合物（LHCI），PSII光捕获复合物（LHCII），细胞色素b_6f复合物（Cyt bf），铁氧还蛋白（Fd）-NADPH氧化还原酶（ FNR），以及在PSII支持条件下来自莱茵衣藻细胞的完整膜蛋白PGRL1（参考文献5）。光谱分析表明，在照明下，来自PSI下游的还原当量被转移到Cyt bf，而被氧化的PSI被来自Cyt bf的还原当量再次还原，表明该超配合物参与了CEF（补充图1）。因此，PSI-LHCI-LHCII-FNR-Cyt bf-PGRL1超复合物的形成和解离不仅控制了两个光系统的能量平衡，而且切换了光合电子流的模式。

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  • 来源
    《Nature》 |2010年第7292期|p.1210-1213|共4页
  • 作者

    Masakazu Iwai; Kenji Takizawa; Ryutaro Tokutsu; Akira Okamuro; Yuichiro Takahashi; Jun Minagawa;

  • 作者单位

    Institute of Low Temperature Science, Hokkaido University, Sapporo 060-0819, Japan Real-Time Bio-Imaging Research Team, Extreme Photonics Research Group, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;

    Institute of Low Temperature Science, Hokkaido University, Sapporo 060-0819, Japan;

    Institute of Low Temperature Science, Hokkaido University, Sapporo 060-0819, Japan;

    Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan;

    Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan;

    Institute of Low Temperature Science, Hokkaido University, Sapporo 060-0819, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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