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The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

机译：CRISPR / Cas细菌免疫系统裂解噬菌体和质粒DNA

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摘要

CRISPR/Cas是一种微生物免疫系统,已知能rn保护细菌不受病毒感染影响.现在研究发现,rn“嗜热链球菌”的CRISPR/Cas系统能通过来rn自病毒和质体的入侵双螺旋DNA的解理来防止rn质体运输和噬菌体感染.该系统似乎非常适应rn这种功能,而且CRISPR/Cas被认为有可能被用rn来自然地生成更安全、更稳健的细菌,它们可rn以抵抗对抗生素有抗性的基因的获取和扩散.%Bacteria and Archaea have developed several defence strategies against foreign nucleic acids such as viral genomes and plasmids. Among them, clustered regularly interspaced short palindromic repeats (CRISPR) loci together with cas (CRISPR-associated) genes form the CRISPR/Cas immune system, which involves partially palindromic repeats separated by short stretches of DNA called spacers, acquired from extrachromosomal elements. It was recently demonstrated that these variable loci can incorporate spacers from infecting bacteriophages and then provide immunity against subsequent bacteriophage infections in a sequence-specific manner. Here we show that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading to plasmid loss. Acquired spacers that match antibiotic-resistance genes provide a novel means to naturally select bacteria that cannot uptake and disseminate such genes. We also provide in vivo evidence that the CRISPRl/Cas system specifically cleaves plasmid and bacteriophage double-stranded DNA within the proto-spacer, at specific sites. Our data show that the CRISPR/Cas immune system is remarkably adapted to cleave invading DNA rapidly and has the potential for exploitation to generate safer microbial strains.
机译：CRISPR/Cas是一种微生物免疫系统,已知能rn保护细菌不受病毒感染影响.现在研究发现,rn“嗜热链球菌”的CRISPR/Cas系统能通过来rn自病毒和质体的入侵双螺旋DNA的解理来防止rn质体运输和噬菌体感染.该系统似乎非常适应rn这种功能,而且CRISPR/Cas被认为有可能被用rn来自然地生成更安全、更稳健的细菌,它们可rn以抵抗对抗生素有抗性的基因的获取和扩散.%Bacteria and Archaea have developed several defence strategies against foreign nucleic acids such as viral genomes and plasmids. Among them, clustered regularly interspaced short palindromic repeats (CRISPR) loci together with cas (CRISPR-associated) genes form the CRISPR/Cas immune system, which involves partially palindromic repeats separated by short stretches of DNA called spacers, acquired from extrachromosomal elements. It was recently demonstrated that these variable loci can incorporate spacers from infecting bacteriophages and then provide immunity against subsequent bacteriophage infections in a sequence-specific manner. Here we show that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading to plasmid loss. Acquired spacers that match antibiotic-resistance genes provide a novel means to naturally select bacteria that cannot uptake and disseminate such genes. We also provide in vivo evidence that the CRISPRl/Cas system specifically cleaves plasmid and bacteriophage double-stranded DNA within the proto-spacer, at specific sites. Our data show that the CRISPR/Cas immune system is remarkably adapted to cleave invading DNA rapidly and has the potential for exploitation to generate safer microbial strains.

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  • 来源
    《Nature》 |2010年第7320期|p.67-71III|共6页
  • 作者

    Josiane E. Garneau; Marie-Eve Dupuis; Manuela Villion; Dennis A. Romero; Rodolphe Barrangou; Patrick Boyaval; Christophe Fremaux; Philippe Horvath; Alfonso H. Magadan; Sylvain Moineau;

  • 作者单位

    Departement de biochimie, de microbiologie et de bio-informatique, Faculte des sciences et de genie, Groupe de recherche en ecologie buccale, Faculte de medecine dentaire. Felix d'Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada;

    rnDepartement de biochimie, de microbiologie et de bio-informatique, Faculte des sciences et de genie, Groupe de recherche en ecologie buccale, Faculte de medecine dentaire. Felix d'Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada;

    rnDepartement de biochimie, de microbiologie et de bio-informatique, Faculte des sciences et de genie, Groupe de recherche en ecologie buccale, Faculte de medecine dentaire. Felix d'Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada;

    rnDanisco USA Inc., 3329 Agriculture Drive, Madison, Wisconsin 53716, USA;

    rnDanisco USA Inc., 3329 Agriculture Drive, Madison, Wisconsin 53716, USA;

    rnDanisco France SAS, Boite Postale 10, F-86220 Dange-Saint-Romain, France;

    rnDanisco France SAS, Boite Postale 10, F-86220 Dange-Saint-Romain, France;

    rnDanisco France SAS, Boite Postale 10, F-86220 Dange-Saint-Romain, France;

    rnDepartement de biochimie, de microbiologie et de bio-informatique, Faculte des sciences et de genie, Groupe de recherche en ecologie buccale, Faculte de medecine dentaire. Felix d'Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada;

    rnDepartement de biochimie, de microbiologie et de bio-informatique, Faculte des sciences et de genie, Groupe de recherche en ecologie buccale, Faculte de medecine dentaire. Felix d'Herelle Reference Center for Bacterial Viruses, Universite Laval, Quebec City, Quebec G1V 0A6, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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