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The active form of DNA polymerase V is UmuD'_2C-RecA-ATP

机译：DNA聚合酶V的活性形式是UmuD'_2C-RecA-ATP

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摘要

DNA-damage-induced SOS mutations arise when Escherichia coli DNA polymerase (pol) V, activated by a RecA nucleoprotein filament (RecA~*), catalyses translesion DNA synthesis. Here we address two longstanding enigmatic aspects of SOS mutagenesis, the molecular composition of mutagenically active pol V and the role of RecA~*. We show that RecA~* transfers a single RecA-ATP stoichiometrically from its DNA 3-end to free pol V (UmuD'_2C) to form an active mutasome (pol V Mut) with the composition UmuD'_2C-RecA-ATP. Pol V Mut catalyses TLS in the absence of RecA~* and deactivates rapidly upon dissociation from DNA. Deactivation occurs more slowly in the absence of DNA synthesis, while retaining RecA-ATP in the complex. Reactivation of pol V Mut is triggered by replacement of RecA-ATP from RecA~*. Thus, the principal role of RecA~* in SOS mutagenesis is to transfer RecA-ATP to pol V, and thus generate active mutasomal complex for translesion synthesis.
机译：当由RecA核蛋白丝（RecA〜*）激活的大肠杆菌DNA聚合酶（pol）V催化跨病变DNA的合成时，就会产生DNA损伤诱导的SOS突变。在这里，我们讨论SOS诱变的两个长期神秘的方面，即诱变活性pol V的分子组成和RecA〜*的作用。我们显示，RecA〜*从其DNA 3端化学计量地将单个RecA-ATP转移至游离pol V（UmuD'_2C），形成具有组成UmuD'_2C-RecA-ATP的活性诱变体（pol V Mut）。在没有RecA〜*的情况下，Pol V Mut可以催化TLS，并在与DNA解离后迅速失活。在没有DNA合成的情况下，失活发生的速度更慢，同时在复合物中保留RecA-ATP。 pol V Mut的重新激活是通过从RecA〜*更换RecA-ATP触发的。因此，RecA〜*在SOS诱变中的主要作用是将RecA-ATP转移到pol V，从而产生用于转移合成的活性诱变复合体。

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  • 来源
    《Nature》 |2009年第7253期|359-363|共5页
  • 作者

    Qingfei Jiang; Kiyonobu Karata; Roger Woodgate; Michael M. Cox; Myron F. Goodman;

  • 作者单位

    Departments of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, California 90089-2910, USA;

    Laboratory of Genomic Integrity,National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-3371, USA;

    Laboratory of Genomic Integrity,National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-3371, USA;

    Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

    Departments of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, California 90089-2910, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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