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Asymptomatic deer excrete infectious prions in faeces

机译:无症状的鹿排出粪便中的感染性ions病毒

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摘要

Infectious prion diseases-scrapie of sheep and chronic wasting disease (CWD) of several species in the deer family-are transmitted naturally within affected host populations. Although several possible sources of contagion have been identified in excretions and secretions from symptomatic animals, the biological importance of these sources in sustaining epidemics remains unclear. Here we show that asymptomatic CWD-infected mule deer (Odocoileus hemionus) excrete CWD prions in their faeces long before they develop clinical signs of prion disease. Intracerebral inoculation of irradiated deer faeces into transgenic mice overexpressing cervid prion protein (PrP) revealed infectivity in 14 of 15 faecal samples collected from five deer at 7-11 months before the onset of neurological disease. Although prion concentrations in deer faeces were considerably lower than in brain tissue from the same deer collected at the end of the disease, the estimated total infectious dose excreted in faeces by an infected deer over the disease course may approximate the total contained in a brain. Prolonged faecal prion excretion by infected deer provides a plausible natural mechanism that might explain the high incidence and efficient horizontal transmission of CWD within deer herds, as well as prion transmission among other susceptible cervids.
机译:感染性ion病毒病-绵羊sc痒症和鹿科中几种物种的慢性消耗病(CWD)-在受影响的寄主种群中自然传播。尽管已从有症状动物的排泄物和分泌物中发现了几种可能的传染源,但这些来源在维持流行病中的生物学重要性仍不清楚。在这里,我们显示无症状的CWD感染的m鹿(Odocoileus hemionus)在他们发展出of病毒疾病的临床体征之前很早就在其粪便中排泄了CWD pr病毒。在大脑中将辐照的鹿粪便接种到过表达宫颈病毒蛋白(PrP)的转基因小鼠中,发现在神经疾病发作之前的7-11个月中,从五只鹿采集的15份粪便样品中有14份具有感染性。尽管在疾病末期收集的同一只鹿的粪便中病毒的浓度大大低于脑组织中的浓度,但在病程中被感染的鹿粪便排泄的估计总传染剂量可能接近大脑中的总浓度。被感染的鹿延长粪便病毒排泄提供了一个合理的自然机制,这可能解释了鹿群内CWD的高发病率和有效水平传播,以及explain病毒在其他易感宫颈中的传播。

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  • 来源
    《Nature》 |2009年第24期|529-532|共4页
  • 作者单位

    Institute for Neurodegenerative Diseases, University of California, San Francisco, California, 94143 USA Department of Neurology, University of California, San Francisco, California, 94143 USA;

    Colorado Division of Wildlife, Wildlife Research Center, Fort Collins, Colorado, 80526 USA;

    Colorado Division of Wildlife, Wildlife Research Center, Fort Collins, Colorado, 80526 USA;

    Colorado Division of Wildlife, Wildlife Research Center, Fort Collins, Colorado, 80526 USA;

    Department of Epidemiology and Biostatistics, University of California, San Francisco, California, 94143 USA;

    Institute for Neurodegenerative Diseases, University of California, San Francisco, California, 94143 USA;

    Department of Pathology, University of California, San Francisco, California, 94143 USA;

    Institute for Neurodegenerative Diseases, University of California, San Francisco, California, 94143 USA Department of Pathology, University of California, 5an Francisco, California, 94143 USA;

    Institute for Neurodegenerative Diseases, University of California, San Francisco, California, 94143 USA Department of Neurology, University of California, San Francisco, California, 94143 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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