• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >Exceptional structured noncoding RNAs revealed by bacterial metagenome analysis
【24h】

Exceptional structured noncoding RNAs revealed by bacterial metagenome analysis

机译：细菌基因组分析显示异常结构化非编码RNA

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Estimates of the total number of bacterial species indicate that existing DNA sequence databases carry only a tiny fraction of the total amount of DNA sequence space represented by this division of life. Indeed, environmental DNA samples have been shown to encode many previously unknown classes of proteins and RNAs. Bioinformatics searches of genomic DNA from bacteria commonly identify new noncoding RNAs (ncRNAs) such as ribo-switches. In rare instances, RNAs that exhibit more extensive sequence and structural conservation across a wide range of bacteria are encountered. Given that large structured RNAs are known to carry out complex biochemical functions such as protein synthesis and RNA processing reactions, identifying more RNAs of great size and intricate structure is likely to reveal additional biochemical functions that can be achieved by RNA. We applied an updated computational pipeline to discover ncRNAs that rival the known large ribozymes in size and structural complexity or that are among the most abundant RNAs in bacteria that encode them. These RNAs would have been difficult or impossible to detect without examining environmental DNA sequences, indicating that numerous RNAs with extraordinary size, structural complexity, or other exceptional characteristics remain to be discovered in unexplored sequence space.
机译：对细菌物种总数的估计表明，现有的DNA序列数据库仅占这种生命划分所代表的DNA序列空间总量的一小部分。实际上，已经证明环境DNA样本编码许多以前未知的蛋白质和RNA类。来自细菌的基因组DNA的生物信息学搜索通常会确定新的非编码RNA（ncRNA），例如核糖开关。在极少数情况下，会遇到在多种细菌中表现出更广泛的序列和结构保守性的RNA。鉴于已知大型结构RNA会执行复杂的生化功能，例如蛋白质合成和RNA加工反应，因此鉴定更多具有大尺寸和复杂结构的RNA可能会揭示RNA可以实现的其他生化功能。我们应用了更新的计算流程，以发现在大小和结构复杂性上可与已知的大型核酶媲美的ncRNA，或在编码它们的细菌中含量最丰富的RNA之一。如果不检查环境DNA序列，将很难或无法检测到这些RNA，这表明在未探索的序列空间中仍有许多具有异常大小，结构复杂性或其他异常特征的RNA仍有待发现。

著录项

  • 来源
    《Nature》 |2009年第7273期|656-659|共4页
  • 作者

    Zasha Weinberg; Jonathan Perreault; Michelle M. Meyer; Ronald R. Breaker;

  • 作者单位

    Howard Hughes Medical Institute, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA Department of Molecular, Cellular and Developmental Biology, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA;

    Department of Molecular, Cellular and Developmental Biology, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA;

    Department of Molecular, Cellular and Developmental Biology, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA;

    Howard Hughes Medical Institute, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA Department of Molecular, Cellular and Developmental Biology, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA Department of Molecular Biophysics and Biochemistry, Yale University, Box 208103, New Haven, Connecticut 06520-8103, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号