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Translation factors promote the formation of two states of the closed-loop mRNP

机译：翻译因子促进闭环mRNP两种状态的形成

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摘要

Efficient translation initiation and optimal stability of most eukar-yotic messenger RNAs depends on the formation of a closed-loop structure and the resulting synergistic interplay between the 5' m~7G cap and the 3' poly(A) tail. Evidence of eIF4G and Pabl interaction supports the notion of a closed-loop mRNP3, but the mechanistic events that lead to its formation and maintenance are still unknown. Here we use toeprinting and polysome profiling assays to delineate ribosome positioning at initiator AUG codons and ribosome-mRNA association, respectively, and find that two distinct stable (resistant to cap analogue) closed-loop structures are formed during initiation in yeast cell-free extracts. The integrity of both forms requires the mRNA cap and poly(A) tail, as well as eIF4E, eIF4G, Pabl and eIF3, and is dependent on the length of both the mRNA and the poly(A) tail. Formation of the first structure requires the 48S ribosomal complex, whereas the second requires an 80S ribosome and the termination factors eRF3/ Sup35 and eRFl/Sup45. The involvement of the termination factors is independent of a termination event.
机译：大多数真核生物信使RNA的有效翻译起始和最佳稳定性取决于闭环结构的形成以及所产生的5'm〜7G帽与3'poly（A）尾部之间的协同相互作用。 eIF4G和Pabl相互作用的证据支持闭环mRNP3的概念，但是导致其形成和维持的机制性事件仍然未知。在这里，我们使用脚印和多核糖体谱分析法分别描绘了核糖体在起始子AUG密码子和核糖体-mRNA缔合处的定位，发现在酵母无细胞提取物中引发过程中形成了两个不同的稳定（对帽类似物有抵抗力）闭环结构。 。两种形式的完整性都需要mRNA帽和poly（A）尾巴，以及eIF4E，eIF4G，Pabl和eIF3，并且取决于mRNA和poly（A）尾巴的长度。第一个结构的形成需要48S核糖体复合物，而第二个结构则需要80S核糖体和终止因子eRF3 / Sup35和eRF1 / Sup45。终止因素的参与与终止事件无关。

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  • 来源
    《Nature》 |2008年第7198期|1276-1280|共5页
  • 作者

    Nadia Amrani; Shubhendu Ghosh; David A. Mangus; Allan Jacobson;

  • 作者单位

    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122, USA;

    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122, USA;

    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122, USA;

    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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