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Promoter-driven splicing regulation in fission yeast

机译：裂变酵母中启动子驱动的剪接调控

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The meiotic cell cycle is modified from the mitotic cell cycle by having a pre-meiotic S phase that leads to high levels of recombination, two rounds of nuclear division with no intervening DNA synthesis and a reductional pattern of chromosome segregation. Reml is a cyclin that is only expressed during meiosis in the fission yeast Schizosaccharomyces pombe. Cells in which reml has been deleted show decreased intragenic meiotic recombination and a delay at the onset of meiosis I (ref. 1). When ectopically expressed in mitotically growing cells, Rem1 induces a G1 arrest followed by severe mitotic catastrophes. Here we show that reml expression is regulated at the level of both transcription and splicing, encoding two proteins with different functions depending on the intron retention. We have determined that the regulation of reml splicing is not dependent on any transcribed region of the gene. Furthermore, when the reml promoter is fused to other intron-containing genes, the chimaeras show a meiotic-specific regulation of splicing, exactly the same as endogenous reml. This regulation is dependent on two transcription factors of the forkhead family, Mei4 (ref. 2) and Fkh2 (ref. 3). Whereas Mei4 induces both transcription and splicing of reml, Fkh2 is responsible for the intron retention of the transcript during vegetative growth and the pre-meiotic S phase.
机译：减数分裂细胞的周期是通过减数分裂前的S期从有丝分裂的细胞周期中改变而来的，该S期可导致高水平的重组，两轮核分裂而无间断的DNA合成以及染色体分离的减少模式。 Reml是一种细胞周期蛋白，仅在减数分裂裂殖酵母粟酒裂殖酵母中减数分裂时表达。 reml缺失的细胞显示出减少的基因内减数分裂重组，减数分裂I发作延迟（参考文献1）。当在有丝分裂生长的细胞中异位表达时，Rem1诱导G1停滞，继而发生严重的有丝分裂灾难。在这里，我们显示reml表达受转录和剪接水平的调节，根据内含子的保留编码两种具有不同功能的蛋白质。我们已经确定reml剪接的调控不依赖于该基因的任何转录区域。此外，当reml启动子与其他含内含子的基因融合时，嵌合体显示出剪接的减数分裂特异性调控，与内源性reml完全相同。该调节取决于叉头家族的两个转录因子Mei4（参考文献2）和Fkh2（参考文献3）。 Mei4诱导reml的转录和剪接，而Fkh2负责营养生长和减数分裂前S期期间转录物的内含子保留。

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