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Deciphering the evolution and metabolism of an anammox bacterium from a community genome

机译:从社区基因组破译厌氧细菌的进化和代谢

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Anaerobic ammonium oxidation (anammox) has become a main focus in oceanography and wastewater treatment. It is also the nitrogen cycle's major remaining biochemical enigma. Among its features, the occurrence of hydrazine as a free intermediate of catabolism, the biosynthesis of ladderane lipids and the role of cytoplasm differentiation are unique in biology. Here we use environmental genomics—the reconstruction of genomic data directly from the environment—to assemble the genome of the uncultured anammox bacterium Kuenenia stuttgartiensis from a complex bioreactor community. The genome data illuminate the evolutionary history of the Planctomycetes and allow us to expose the genetic blueprint of the organism's special properties. Most significantly, we identified candidate genes responsible for ladderane biosynthesis and biological hydrazine metabolism, and discovered unexpected metabolic versatility.
机译:厌氧铵氧化(厌氧氨)已成为海洋学和废水处理的主要重点。它也是氮循环中主要的剩余生化谜团。在其特征中,肼作为分解代谢的自由中间体的出现,梯丹脂质的生物合成以及细胞质分化的作用在生物学中是独特的。在这里,我们使用环境基因组学(直接从环境中重建基因组数据)来组装来自复杂生物反应器群落的未培养的厌氧氨氧化细菌斯图加特犬的基因组。基因组数据阐明了扁平菌的进化历史,并使我们能够揭示生物特殊特性的遗传蓝图。最重要的是,我们确定了负责梯形生物合成和生物肼代谢的候选基因,并发现了意想不到的代谢多功能性。

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