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Gene transfer to plants by diverse species of bacteria

机译：基因通过多种细菌转移到植物

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摘要

Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology. However, the complexity of the patent landscape has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation.
机译：农杆菌被广泛认为是唯一能够将基因转移至植物的细菌属。经过适当的修饰，农杆菌已成为植物生物技术中最有效的基因转移载体。但是，专利格局的复杂性为全球许多公共和私人组织有效地使用该技术进行农业改良创造了实际和可感知的障碍。在这里我们表明，农杆菌属以外的几种细菌可以被修饰以介导基因转移到许多不同的植物上。通过获得解除武装的Ti质粒和合适的二元载体，使这些与植物相关的共生细菌能够胜任基因转移。除了为植物生物技术提供通用的“开源”平台以外，这种替代农杆菌介导的技术来改善作物的方法还可能导致天然细菌与植物相互作用的新用途，以实现植物转化。

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