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Identification of the pollen determinant of S-RNase-mediated self-incompatibility

机译：S-RNase介导的自交不亲和性的花粉决定因素的鉴定

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摘要

Many flowering plants have adopted self-incompatibility mechanisms to prevent inbreeding and promote out-crosses(1). In the Solanaceae, Rosaceae and Scrophulariaceae, two separate genes at the highly polymorphic S-locus control self-incompatibility interactions: the S-RNase gene encodes the pistil determinant and the previously unidentified S-gene encodes the pollen determinant(2-4). S-RNases interact with pollen S-allele products to inhibit the growth of self-pollen tubes in the style. Pollen-expressed F-box genes showing allelic sequence polymorphism have recently been identified near to the S-RNase gene in members of the Rosaceae and Scrophulariaceae(5-8); but until now have not been directly shown to encode the pollen determinant. Here we report the identification and characterization of PiSLF, an S-locus F-box gene of Petunia inflata ( Solanaceae). We show that transformation of S1S1, S1S2 and S2S3 plants with the S-2-allele of PiSLF causes breakdown of their pollen function in self-incompatibility. This breakdown of pollen function is consistent with 'competitive interaction', in which pollen carrying two different pollen S-alleles fails to function in self-incompatibility(1,9,10). We conclude that PiSLF encodes the pollen self-incompatibility determinant.
机译：许多开花植物已经采用了自交不亲和机制来防止近交和促进杂交（1）。在茄科，蔷薇科和玄参科中，高度多态的S-基因座处有两个独立的基因控制自我不相容性相互作用：S-RNase基因编码雌蕊决定簇，而先前未鉴定的S基因编码花粉决定簇（2-4）。 S-RNase与花粉S-等位基因产物相互作用，以抑制自花粉管的生长。最近发现，在蔷薇科和玄参科的S-RNase基因附近已发现花粉表达的F-box基因具有等位基因序列多态性（5-8）；但是到目前为止，还没有直接显示出编码花粉决定簇的信息。在这里，我们报告鉴定和鉴定PiSLF，矮牵牛（茄科）的S-基因座F-box基因。我们显示，PiSLF的S-2-等位基因对S1S1，S1S2和S2S3植物的转化会导致其花粉功能在自交不亲和性方面崩溃。花粉功能的这种破坏与“竞争性相互作用”相一致，其中携带两个不同花粉S-等位基因的花粉不能自我不相容（1,9,10）。我们得出结论，PiSLF编码花粉自交不亲和性的决定因素。

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