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首页> 外文期刊>Nature >Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
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Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris

机译：20个小鼠器官的单细胞转录组学创建了Tabula Muris

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摘要

Here we present a compendium of single-cell transcriptomic data from the model organism Mus musculus that comprises more than 100,000 cells from 20 organs and tissues. These data represent a new resource for cell biology, reveal gene expression in poorly characterized cell populations and enable the direct and controlled comparison of gene expression in cell types that are shared between tissues, such as T lymphocytes and endothelial cells from different anatomical locations. Two distinct technical approaches were used for most organs: one approach, microfluidic droplet-based 3'-end counting, enabled the survey of thousands of cells at relatively low coverage, whereas the other, full-length transcript analysis based on fluorescence-activated cell sorting, enabled the characterization of cell types with high sensitivity and coverage. The cumulative data provide the foundation for an atlas of transcriptomic cell biology.
机译：在这里，我们介绍了来自模型生物小家鼠的单细胞转录组学数据汇编，其中包括来自20个器官和组织的100,000多个细胞。这些数据代表了细胞生物学的新资源，揭示了表征欠佳的细胞群体中的基因表达，并能够直接和受控地比较组织之间共享的细胞类型（例如，来自不同解剖位置的T淋巴细胞和内皮细胞）中的基因表达。大多数器官使用两种截然不同的技术方法：一种方法是基于微流体液滴的3'末端计数，从而能够以相对较低的覆盖率对数千个细胞进行调查，而另一种方法是基于荧光激活的细胞进行全长转录本分析通过分选，可以以高灵敏度和覆盖率表征细胞类型。累积数据为转录组细胞生物学地图集提供了基础。

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  • 来源
    《Nature》 |2018年第7727期|367-372|共6页
  • 作者

    Schaum Nicholas; Karkanias Jim; Neff Norma F.; May Andrew P.; Quake Stephen R.; Wyss-Coray Tony; Darmanis Spyros; Batson Joshua; Botvinnik Olga; Chen Michelle B.; Chen Steven; Green Foad; Jones Robert C.; Maynard Ashley; Penland Lolita; Pisco Angela Oliveira; Sit Rene V.; Stanley Geoffrey M.; Webber James T.; Zanini Fabio; Baghel Ankit S.; Bakerman Isaac; Bansal Ishita; Berdnik Daniela; Bilen Biter; Brownfield Douglas; Cain Corey; Cho Min; Cirolia Giana; Conley Stephanie D.; Demers Aaron; Demir Kubilay; de Morree Antoine; Divita Tessa; du Bois Haley; Dulgeroff Laughing Bear Torrez; Ebadi Hamid; Espinoza F. Hernan; Fish Matt; Gan Qiang; George Benson M.; Gillich Astrid; Genetiano Geraldine; Gu Xueying; Gulati Gunsagar S.; Hang Yan; Hosseinzadeh Shayan; Huang Albin; Iram Tal; Isobe Taichi; Ives Feather; Kao Kevin S.; Karnam Guruswamy; Kershner Aaron M.; Kiss Bernhard M.; Kong William; Kumar Maya E.; Lam Jonathan Y.; Lee Davis P.; Lee Song E.; Li Guang; Li Qingyun; Liu Ling; Lo Annie; Lu Wan-Jin; Manjunath Anoop; May Kaia L.; May Oliver L.; McKay Marina; Metzger Ross J.; Mignardi Marco; Min Dullei; Nabhan Ahmad N.; Ng Katharine M.; Noh Joseph; Patkar Rasika; Peng Weng Chuan; Puccinelli Robert; Sikandar Shaheen S.; Sinha Rahul; Szade Krzysztof; Tan Weilun; Tato Cristina; Tellez Krissie; Travaglini Kyle J.; Tropini Carolina; Waldburger Lucas; van Weele Linda J.; Wosczyna Michael N.; Xiang Jinyi; Xue Soso; Youngyunpipatkul Justin; Zardeneta Macy E.; Zhang Fan; Zhou Lu; Castro Paola; Croote Derek; DeRisi Joseph L.; Kuo Christin S.; Lehallier Benoit; Nguyen Patricia K.; Tan Serena Y.; Wang Bruce M.; Yousef Hanadie; Wyss-Coray Tony; Beachy Philip A.; Chan Charles K. F.; Wang Bruce M.; Weinberg Kenneth; Wu Sean M.; Yousef Hanadie; Barres Ben A.; Chan Charles K. F.; Clarke Michael F.; Huang Kerwyn Casey; Kim Seung K.; Krasnow Mark A.; Nusse Roel; Rando Thomas A.; Sonnenburg Justin; Weissman Irving L.; Wu Sean M.;

  • 作者单位

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    VA Palo Alto Healthcare Syst, Flow Cytometry Core, Palo Alto, CA USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Sean N Parker Ctr Asthma & Allergy Res, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA;

    VA Palo Alto Healthcare Syst, Ctr Tissue Regenerat Repair & Restorat, Palo Alto, CA 94304 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Div Cardiovasc Med, Dept Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurobiol, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Vera Moulton Wall Ctr Pulm & Vasc Dis, Stanford, CA 94305 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Pulm Med, Dept Pediat, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA;

    Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    VA Palo Alto Healthcare Syst, Ctr Tissue Regenerat Repair & Restorat, Palo Alto, CA 94304 USA;

    Stanford Univ, Sch Med, Vera Moulton Wall Ctr Pulm & Vasc Dis, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurobiol, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA;

    Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Div Plast & Reconstruct Surg, Dept Surg, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Pulm Med, Dept Pediat, Stanford, CA 94305 USA;

    Stanford Univ, Div Cardiovasc Med, Dept Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurobiol, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA;

    Chan Zuckerberg Biohub, San Francisco, CA 94158 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

    Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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