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Plant 22-nt siRNAs mediate translational repression and stress adaptation

机译：植物22-nt siRNA介导翻译抑制和逆境适应

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Characterization of 22-nucleotide short interfering RNAs in plants finds that they accumulate in response to environmental stress, causing translational repression, inhibition of plant growth and enhanced stress responses.Small interfering RNAs (siRNAs) are essential for proper development and immunity in eukaryotes(1). Plants produce siRNAs with lengths of 21, 22 or 24 nucleotides. The 21- and 24-nucleotide species mediate cleavage of messenger RNAs and DNA methylation(2,3), respectively, but the biological functions of the 22-nucleotide siRNAs remain unknown. Here we report the identification and characterization of a group of endogenous 22-nucleotide siRNAs that are generated by the DICER-LIKE 2 (DCL2) protein in plants. When cytoplasmic RNA decay and DCL4 are deficient, the resulting massive accumulation of 22-nucleotide siRNAs causes pleiotropic growth disorders, including severe dwarfism, meristem defects and pigmentation. Notably, two genes that encode nitrate reductases-NIA1 and NIA2-produce nearly half of the 22-nucleotide siRNAs. Production of 22-nucleotide siRNAs triggers the amplification of gene silencing and induces translational repression both gene specifically and globally. Moreover, these 22-nucleotide siRNAs preferentially accumulate upon environmental stress, especially those siRNAs derived from NIA1/2, which act to restrain translation, inhibit plant growth and enhance stress responses. Thus, our research uncovers the unique properties of 22-nucleotide siRNAs, and reveals their importance in plant adaptation to environmental stresses.
机译：对植物中22个核苷酸的短干扰RNA进行鉴定后发现，它们会在环境胁迫下积聚，从而引起翻译抑制，抑制植物生长并增强胁迫响应。小干扰RNA（siRNA）对于真核生物的正常发育和免疫力至关重要（1 ）。植物产生长度为21、22或24个核苷酸的siRNA。 21和24个核苷酸的物种分别介导信使RNA的裂解和DNA甲基化（2,3），但22个核苷酸的siRNA的生物学功能仍然未知。在这里我们报告鉴定和表征一组由植物中的DICER-LIKE 2（DCL2）蛋白产生的内源22个核苷酸的siRNA。当胞质RNA衰变和DCL4不足时，由此形成的22个核苷酸的siRNA大量积累会引起多效性生长障碍，包括严重的侏儒症，分生组织缺陷和色素沉着。值得注意的是，编码硝酸盐还原酶NIA1和NIA2的两个基因产生了将近22个核苷酸的siRNA的一半。产生22个核苷酸的siRNA会触发基因沉默的扩增，并导致基因特异性和全局性的翻译抑制。此外，这些22个核苷酸的siRNA优先在环境胁迫下积累，尤其是那些源自NIA1 / 2的siRNA，它们起着抑制翻译，抑制植物生长和增强胁迫反应的作用。因此，我们的研究揭示了22个核苷酸的siRNA的独特特性，并揭示了它们在植物适应环境胁迫中的重要性。

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    《Nature》 |2020年第7806期|89-93|共5页
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    Southern Univ Sci & Technol Dept Biol Inst Plant & Food Sci Shenzhen Peoples R China|Peking Univ Sch Life Sci State Key Lab Prot & Plant Gene Res Beijing Peoples R China;

    Southern Univ Sci & Technol Dept Biol Inst Plant & Food Sci Shenzhen Peoples R China;

    Univ Tokyo Inst Quantitat Biosci Tokyo Japan|Japan Sci & Technol Agcy JST Precursory Res Embryon Sci & Technol PRESTO Saitama Japan;

    Southern Univ Sci & Technol Dept Biol Inst Plant & Food Sci Shenzhen Peoples R China|Northeast Forestry Univ Key Lab Plant Ecol Harbin Peoples R China;

    Southern Univ Sci & Technol Dept Biol Inst Plant & Food Sci Shenzhen Peoples R China|Beijing Forestry Univ Beijing Adv Innovat Ctr Tree Breeding Mol Design Natl Engn Lab Tree Breeding Coll Biol Sci & Technol Beijing Peoples R China;

    Peking Univ Sch Life Sci State Key Lab Prot & Plant Gene Res Beijing Peoples R China|Chinese Acad Agr Sci Lingnan Guangdong Lab Modern Agr Genome Anal Lab Minist Agr Agr Genom Inst Shenzhen Shenzhen Peoples R China;

    Northeast Forestry Univ Key Lab Plant Ecol Harbin Peoples R China;

    Beijing Forestry Univ Beijing Adv Innovat Ctr Tree Breeding Mol Design Natl Engn Lab Tree Breeding Coll Biol Sci & Technol Beijing Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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