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Genetic dissection of the glutamatergic neuron system in cerebral cortex

机译：脑皮层谷氨酸神经元系统的遗传解剖

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摘要

Diverse types of glutamatergic pyramidal neurons mediate the myriad processing streams and output channels of the cerebral cortex~(1,2), yet all derive from neural progenitors of the embryonic dorsal telencephalon~(3,4). Here we establish genetic strategies and tools for dissecting and fate-mapping subpopulations of pyramidal neurons on the basis of their developmental and molecular programs. We leverage key transcription factors and effector genes to systematically target temporal patterning programs in progenitors and differentiation programs in postmitotic neurons. We generated over a dozen temporally inducible mouse Cre and Flp knock-in driver lines to enable the combinatorial targeting of major progenitor types and projection classes. Combinatorial strategies confer viral access to subsets of pyramidal neurons defined by developmental origin, marker expression, anatomical location and projection targets. These strategies establish an experimental framework for understanding the hierarchical organization and developmental trajectory of subpopulations of pyramidal neurons that assemble cortical processing networks and output channels.
机译：不同类型的谷氨酸谷氨酸金字塔神经元介导脑皮质〜（1,2）的无数加工流和输出通道，但所有这些都来自胚胎背部瞳孔〜（3,4）的神经祖细胞。在这里，我们在其发育和分子计划的基础上建立遗传策略和用于解剖和命运锥体神经元的群体的工具。我们利用关键转录因子和效应基因来系统地靶向祖先神经元祖细胞和分化方案的颞型图案化程序。我们在十几个时间诱导的鼠标CRE和FLP敲击驱动线上产生，以实现主要祖先类型和投影类的组合靶向。组合策略赋予了由发育来源，标记表达，解剖学位置和投影目标定义的金字塔神经元的病毒进入。这些策略建立了一个实验框架，用于了解组织的分层组织和群体神经元的群体的发展轨迹，这些锥体神经元组装皮质处理网络和输出通道。

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  • 来源
    《Nature》 |2021年第7879期|182-187|共6页
  • 作者

    Katherine S Matho; Dhananjay Huilgol; William Galbavy; Miao He; Gukhan Kim; Xu An; Jiangteng Lu; Priscilla Wu; Daniela J Di Bella; Ashwin S Shetty; Ramesh Palaniswamy; Joshua Hatfield; Ricardo Raudales; Arun Narasimhan; Eric Gamache; Jesse M Levine; Jason Tucciarone; Eric Szelenyi; Julie A Harris; Partha P Mitra; Pavel Osten; Paola Arlotta; Z Josh Huang;

  • 作者单位

    Cold Spring Harbor Laboratory;

    Cold Spring Harbor Laboratory|Department of Neurobiology Duke University Medical Center;

    Cold Spring Harbor Laboratory|Program in Neuroscience Department of Neurobiology and Behavior Stony Brook University;

    Cold Spring Harbor Laboratory|Institutes of Brain Science State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan University;

    Cold Spring Harbor Laboratory;

    Cold Spring Harbor Laboratory|Department of Neurobiology Duke University Medical Center;

    Cold Spring Harbor Laboratory|Shanghai Jiaotong University Medical School;

    Cold Spring Harbor Laboratory;

    Department of Stem Cell and Regenerative Biology Harvard University;

    Department of Stem Cell and Regenerative Biology Harvard University;

    Cold Spring Harbor Laboratory;

    Cold Spring Harbor Laboratory|Department of Neurobiology Duke University Medical Center;

    Cold Spring Harbor Laboratory|Program in Neuroscience Department of Neurobiology and Behavior Stony Brook University;

    Cold Spring Harbor Laboratory;

    Cold Spring Harbor Laboratory;

    Cold Spring Harbor Laboratory|Program in Neuroscience and Medical Scientist Training Program Stony Brook University;

    Cold Spring Harbor Laboratory|Program in Neuroscience and Medical Scientist Training Program Stony Brook University|Department of Psychiatry Stanford University School of Medicine;

    Cold Spring Harbor Laboratory;

    Program in Neuroscience and Medical Scientist Training Program Stony Brook University|Allen Institute for Brain Science;

    Cold Spring Harbor Laboratory;

    Cold Spring Harbor Laboratory;

    Department of Stem Cell and Regenerative Biology Harvard University|Stanley Center for Psychiatric Research;

    Cold Spring Harbor Laboratory|Department of Neurobiology Duke University Medical Center;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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