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首页> 外文期刊>Nature >COPII-cargo interactions direct protein sorting into ER-derived transport vesicles.
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COPII-cargo interactions direct protein sorting into ER-derived transport vesicles.

机译：COPII货物相互作用直接将蛋白质分类到ER衍生的运输小泡中。

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摘要

Vesicles coated with coat protein complex II (COPII) selectively transport molecules (cargo) and vesicle fusion proteins from the endoplasmic reticulum (ER) to the Golgi complex. We have investigated the role of coat proteins in cargo selection and recruitment. We isolated integral membrane and soluble cargo proteins destined for transport from the ER in complexes formed in the presence of Sar1 and Sec23/24, a subset of the COPII components, and GTP or GMP-PNP. Vesicle fusion proteins of the vSNARE family and Emp24, a member of a putative cargo carrier family, were also found in COPII complexes. The inclusion of amino-acid permease molecules into the complex depended on the presence of Shr3, a protein required for the permease to leave the ER. Resident ER proteins Sec61, BiP (Kar2) and Shr3 were not included in the complexes, indicating that the COPII components bound specifically to vesicle cargo. COPII-cargo complexes and putative cargo adaptor-cargo complexes were also isolated from COPII vesicles. Our results indicate that cargo packaging signals and soluble cargo adaptors are recognized by a recruitment complex comprising Sar1-GTP and Sec23/24.
机译：涂有外壳蛋白复合物II（COPII）的囊泡选择性地将分子（货物）和囊泡融合蛋白从内质网（ER）转运到高尔基体。我们研究了外壳蛋白在货物选择和招募中的作用。我们分离了在SARII和Sec23 / 24（COPII成分的一个子集）和GTP或GMP-PNP的存在下形成的复合物中注定要从ER运输的完整膜和可溶性货物蛋白。在COPII复合物中还发现了vSNARE家族和Emp24（一种假定的货物载体家族的成员）的囊泡融合蛋白。氨基酸渗透酶分子是否包含在复合物中取决于Shr3的存在，Shr3是渗透酶离开ER所需的蛋白质。驻留的ER蛋白Sec61，BiP（Kar2）和Shr3没有包含在复合物中，这表明COPII成分与小泡货物特异性结合。 COPII-货物复合物和推定的货物衔接子-货物复合物也从COPII囊泡中分离出来。我们的结果表明，包括Sar1-GTP和Sec23 / 24的募集复合物可以识别货物包装信号和可溶性货物适配器。

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