Trans-activation of group Ⅱ intron splicing by nuclear U5 snRNA

Martin Hetzer; Gabriele Wurzer; Rudolf J. Schweyen; Manfred W. Mueller

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Trans-activation of group Ⅱ intron splicing by nuclear U5 snRNA

机译：U5 snRNA对Ⅱ组内含子剪接的反式激活

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Similarities between RNA splicing during autocatalytic excision of group Ⅱ introns and pre-mRNA processing led to the hypothesis that group Ⅱ introns might be the evolutionary predecessors of spliceosomal small nuclear RNAs. The ID3 subdomain stem-loop structure of group Ⅱ introns, the proposed analogue of the spliceosomal U5 snRNA, is thought to be essential for 5′ splice site recognition and anchoring of the free 5′ exon. Using the group Ⅱ intron bI1 we have analysed the role of ID3 in splicing. In the absence of ID3 the 5′ splice site was recognized accurately and efficiently, but exon anchoring was greatly reduced. This step was restored in the presence of RNA fragments consisting of either the terminal stem-loop structure of ID3 or spliceosomal U5 snRNA. This suggests that the predominant role of both RNAs is to anchor the 5′ exon during exon ligation. Furthermore, as U5 complements for the loss of ID3, a similar network of structural RNAs may form the catalytic core of both group Ⅱ introns and spliceo-somes.

机译：Ⅱ组内含子自催化切除过程中的RNA剪接与前mRNA加工之间的相似性导致了这样一个假设，即Ⅱ组内含子可能是剪接小核RNA的进化前体。 Ⅱ类内含子的ID3亚域茎环结构是剪接体U5 snRNA的拟议类似物，被认为对5'剪接位点的识别和游离5'外显子的锚定至关重要。使用Ⅱ组内含子bI1，我们分析了ID3在剪接中的作用。在没有ID3的情况下，可以准确，有效地识别5'剪接位点，但显着减少了外显子锚定。在存在由ID3的末端茎环结构或剪接的U5 snRNA组成的RNA片段的情况下恢复该步骤。这表明两种RNA的主要作用是在外显子连接过程中锚定5'外显子。此外，由于U5弥补了ID3的缺失，类似的结构RNA网络可能形成Ⅱ组内含子和剪接体的催化核心。

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《Nature》 |1997年第6623期|p.417-420|共4页
作者
Martin Hetzer; Gabriele Wurzer; Rudolf J. Schweyen; Manfred W. Mueller;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. A conditional U5 snRNA mutation affecting pre-mRNA splicing and nuclear pre-mRNA retention identifies SSD1/SRK1 as a general splicing mutant suppressor. [J] . Luukkonen BG, Seraphin B Nucleic Acids Research . 1999,第17期

机译：有条件的U5 snRNA突变会影响前mRNA的剪接和核前mRNA的保留，从而将SSD1 / SRK1识别为一般的剪接突变体抑制子。
2. A conditional U5 snRNA mutation affecting pre-mRNA splicing and nuclear pre-mRNA retention identifies SSD1/SRK1 as a general splicing mutant suppressor [J] . B.G. Mattias Luukkonen, Bertrand Séraphin Nucleic acids research . 1999,第17期

机译：有条件的U5 snRNA突变会影响前mRNA的剪接和核前mRNA的保留，将SSD1 / SRK1识别为一般的剪接突变体抑制子
3. REQUIREMENT OF U12 SNRNA FOR IN VIVO SPLICING OF A MINOR CLASS OF EUKARYOTIC NUCLEAR PRE-MRNA INTRONS [J] . Hall SL, Padgett RA Science . 1996,第5256期

机译：U12 SNRNA体内拼接微小核真核mRNA前体的需求
4. Splice site pattern analysis and identification of similar sequences in the deep intron areas of human chromosome 21 [C] . Cristian G. Zimbru, Nicoleta Andreescu, Adela Chirita-Emandi, 2017 E-Health and Bioengineering Conference . 2017

机译：人类21号染色体深内含子区域的剪接位点模式分析和相似序列的鉴定
5. Biochemical characterization of bacterial group II introns: Rules for 3' splice site selection and insertion of IIC introns after intrinsic terminator motifs [D] . Robart, Aaron Reigh 2007

机译：细菌第二组内含子的生化特性：固有终止子基序后3'剪接位点选择和IIC内含子插入的规则
6. Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing [O] . Timothy S. McConnell, Joan A. Steitz 2001

机译：前mRNA剪接过程中U5 snRNA的恒定环与第二个内含子残基的接近度
7. A conditional U5 snRNA mutation affecting pre-mRNA splicing and nuclear pre-mRNA retention identifies SSD1/SRK1 as a general splicing mutant suppressor. [O] . Luukkonen, B G, Séraphin, B 1999

机译：有条件的U5 snRNA突变会影响前mRNA剪接和核前mRNA保留，从而将SSD1 / SRK1识别为一般的剪接突变体抑制子。

Trans-activation of group Ⅱ intron splicing by nuclear U5 snRNA

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