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首页> 外文期刊>Nature >Specific and redundant roles of Src and Fyn in organizing the cytoskeleton.
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Specific and redundant roles of Src and Fyn in organizing the cytoskeleton.

机译：Src和Fyn在组织细胞骨架中的特定和冗余作用。

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摘要

Mouse embryos lacking Csk, a negative regulator of Src family kinases, exhibit defects in neurulation and die at mid-gestation. To determine the role of activated Src family kinases in the csk- phenotype, we have introduced mutations in the src and fyn genes into the csk- mutant background. Genetic analysis reveals that src, but not fyn, is partly epistatic to the csk gene. Biochemical analysis indicates that several cytoskeletal proteins are hyperphosphorylated on tyrosine residues in csk- cells. Regulation of cortactin and tensin hyperphosphorylation is Src-dependent, whereas focal adhesion kinase and paxillin hyperphosphorylation is partly dependent on both Src and Fyn. Furthermore, the src- mutation can restore the normal distribution of cortactin and partly correct filamentous actin organization in csk-cells. Thus, Src family kinases have both specific and overlapping functions in regulation of the cytoskeleton. The disturbance of these functions may be a molecular basis for the phenotype exhibited by csk- mutants.
机译：缺少Csk（Src家族激酶的负调节剂）的小鼠胚胎在神经中表现出缺陷，并在妊娠中期死亡。为了确定活化的Src家族激酶在csk表型中的作用，我们将src和fyn基因的突变引入了csk突变背景中。遗传分析表明，src而非cyn对csk基因部分上位。生化分析表明，几种细胞骨架蛋白在csk细胞中的酪氨酸残基上被过度磷酸化。皮质激素和张力蛋白的过度磷酸化的调节依赖于Src，而粘着斑激酶和paxillin的过度磷酸化则部分依赖于Src和Fyn。此外，src突变可恢复cortactin的正常分布并部分纠正csk细胞中的丝状肌动蛋白组织。因此，Src家族激酶在调节细胞骨架中既具有特异性功能又具有重叠功能。这些功能的紊乱可能是csk突变体表现出的表型的分子基础。

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