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首页> 外文期刊>Ocean Science Journal >Assessment of immune parameters of manila clam Ruditapes philippinarum in different physiological conditions using flow cytometry
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Assessment of immune parameters of manila clam Ruditapes philippinarum in different physiological conditions using flow cytometry

机译：流式细胞术评估菲律宾蛤仔菲律宾蛤仔在不同生理条件下的免疫参数

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摘要

Cellular and humoral immune parameters are often used as biomarkers to trace environmental and physiological stresses in marine bivalves. In this study, we compared various immune parameters of Manila clams (Ruditapes philippinarum) under normal conditions and under a high level of desiccation, using flow cytometry. The immune parameters analyzed included, total hemocyte count, hemocyte mortality, hemocyte DNA damage, reactive oxygen species (ROS) production, and phagocytosis activity. Total hemocyte count, hemocyte DNA damage, and hemocyte mortality were significantly elevated among clams under high desiccation stress, while phagocytosis activity and spontaneous ROS production were significantly lower compared to those parameters of the control clams (p<0.05). These data suggest that the immune parameters analyzed in this study well reflect the physiological status of clams.
机译：细胞和体液免疫参数通常用作生物标记物，以追踪海洋双壳类动物的环境和生理压力。在这项研究中，我们使用流式细胞术比较了马尼拉蛤（菲律宾蛤仔）在正常条件下和高度干燥下的各种免疫参数。分析的免疫参数包括总血细胞计数，血细胞死亡率，血细胞DNA损伤，活性氧（ROS）产生和吞噬活性。在高干燥压力下，蛤的总血细胞计数，血细胞DNA损伤和血细胞死亡率均显着升高，而吞噬作用活性和自发ROS生成则显着低于对照蛤的参数（p <0.05）。这些数据表明，本研究中分析的免疫参数很好地反映了蛤的生理状态。

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